两种市售蜡样芽孢杆菌腹泻性肠毒素免疫分析试剂盒所检测抗原的鉴定与分析
Identification and analysis of the antigens detected by two commercial Bacillus cereus diarrheal enterotoxin immunoassay kits.
作者信息
Beecher D J, Wong A C
机构信息
Department of Food Microbiology and Toxicology, University of Wisconsin, Madison 53706.
出版信息
Appl Environ Microbiol. 1994 Dec;60(12):4614-6. doi: 10.1128/aem.60.12.4614-4616.1994.
The usefulness of two commercial immunoassays for the detection of diarrheal enterotoxin of Bacillus cereus is unclear because the identity of the enterotoxin(s) has not been proven and the kits detect different proteins. We found that the Bacillus cereus Enterotoxin-Reversed Passive Latex Agglutination kit (Oxoid) detects the L2 component from hemolysin BL, and the Bacillus Diarrhoeal Enterotoxin Visual Immunoassay (Tecra) detects two apparently nontoxic proteins.
两种用于检测蜡样芽孢杆菌腹泻性肠毒素的商业免疫测定法的效用尚不清楚，因为肠毒素的身份尚未得到证实，而且这些试剂盒检测的是不同的蛋白质。我们发现，蜡样芽孢杆菌肠毒素反向被动乳胶凝集试剂盒（Oxoid）检测的是溶血素BL中的L2成分，而蜡样芽孢杆菌腹泻性肠毒素视觉免疫测定法（Tecra）检测的是两种明显无毒的蛋白质。
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