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两种市售蜡样芽孢杆菌腹泻性肠毒素免疫分析试剂盒所检测抗原的鉴定与分析

Identification and analysis of the antigens detected by two commercial Bacillus cereus diarrheal enterotoxin immunoassay kits.

作者信息

Beecher D J, Wong A C

机构信息

Department of Food Microbiology and Toxicology, University of Wisconsin, Madison 53706.

出版信息

Appl Environ Microbiol. 1994 Dec;60(12):4614-6. doi: 10.1128/aem.60.12.4614-4616.1994.

DOI:10.1128/aem.60.12.4614-4616.1994
PMID:7811099
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC202031/
Abstract

The usefulness of two commercial immunoassays for the detection of diarrheal enterotoxin of Bacillus cereus is unclear because the identity of the enterotoxin(s) has not been proven and the kits detect different proteins. We found that the Bacillus cereus Enterotoxin-Reversed Passive Latex Agglutination kit (Oxoid) detects the L2 component from hemolysin BL, and the Bacillus Diarrhoeal Enterotoxin Visual Immunoassay (Tecra) detects two apparently nontoxic proteins.

摘要

两种用于检测蜡样芽孢杆菌腹泻性肠毒素的商业免疫测定法的效用尚不清楚，因为肠毒素的身份尚未得到证实，而且这些试剂盒检测的是不同的蛋白质。我们发现，蜡样芽孢杆菌肠毒素反向被动乳胶凝集试剂盒（Oxoid）检测的是溶血素BL中的L2成分，而蜡样芽孢杆菌腹泻性肠毒素视觉免疫测定法（Tecra）检测的是两种明显无毒的蛋白质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/499b/202031/e3450646bda9/aem00029-0409-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/499b/202031/e3450646bda9/aem00029-0409-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/499b/202031/e3450646bda9/aem00029-0409-a.jpg

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本文引用的文献

1
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Appl Environ Microbiol. 1994 Jun;60(6):1719-25. doi: 10.1128/aem.60.6.1719-1725.1994.
2
A large outbreak of gastroenteritis caused by diarrheal toxin-producing Bacillus cereus.
J Infect Dis. 1993 Jun;167(6):1452-5. doi: 10.1093/infdis/167.6.1452.
3
Analysis of enterotoxin production by Bacillus cereus from dairy products, food poisoning incidents and non-gastrointestinal infections.对来自乳制品、食物中毒事件及非胃肠道感染的蜡样芽孢杆菌产生肠毒素的分析。
Toxins (Basel). 2021 Jan 28;13(2):98. doi: 10.3390/toxins13020098.
4
Charge-transfer interaction of drug quinidine with quinol, picric acid and DDQ: Spectroscopic characterization and biological activity studies towards understanding the drug-receptor mechanism.药物奎尼丁与喹啉、苦味酸和2,3-二氯-5,6-二氰基-1,4-苯醌的电荷转移相互作用：用于理解药物-受体机制的光谱表征和生物活性研究
J Pharm Anal. 2014 Apr;4(2):81-95. doi: 10.1016/j.jpha.2013.06.003. Epub 2013 Jul 10.
5
Characterization of LysB4, an endolysin from the Bacillus cereus-infecting bacteriophage B4.LysB4 的特性研究，一种来自于感染蜡样芽孢杆菌的噬菌体 B4 的溶菌酶。
BMC Microbiol. 2012 Mar 15;12:33. doi: 10.1186/1471-2180-12-33.
6
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BMC Microbiol. 2010 Nov 30;10:304. doi: 10.1186/1471-2180-10-304.
7
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4
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5
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FEMS Microbiol Lett. 1993 Jun 1;110(1):97-100. doi: 10.1111/j.1574-6968.1993.tb06301.x.
6
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7
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8
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9
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10
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Infect Immun. 1984 Mar;43(3):887-94. doi: 10.1128/iai.43.3.887-894.1984.