Dover R, Jayaram Y, Patel K, Chinery R
Department of Anatomy, Queen Mary and Westfield College, University of London, UK.
J Cell Sci. 1994 May;107 ( Pt 5):1181-4. doi: 10.1242/jcs.107.5.1181.
p53 inhibits division following cellular damage. Cultured cells were found to express p53 protein following pulse labelling with radioisotopes, even at low doses normally used for growth and metabolic labelling studies. Some stem cells are exquisitely sensitive to radiation and thus p53 may have evolved as a major regulator of stem cell function. Therefore any genetic damage may be able to induce p53 expression, which in turn will affect the biochemical outcome of many experiments by both cell cycle arrest and other mechanisms. In some cases the use of radioisotopes may directly change the results of the experiment. This will require a careful re-evaluation of the current literature and experimental protocols utilising radioisotopes.
p53在细胞受损后抑制细胞分裂。在用放射性同位素进行脉冲标记后，发现培养的细胞会表达p53蛋白，即使是在通常用于生长和代谢标记研究的低剂量下。一些干细胞对辐射极其敏感，因此p53可能已进化成为干细胞功能的主要调节因子。所以，任何基因损伤都可能诱导p53表达，而这反过来又会通过细胞周期停滞和其他机制影响许多实验的生化结果。在某些情况下，使用放射性同位素可能会直接改变实验结果。这将需要对当前使用放射性同位素的文献和实验方案进行仔细的重新评估。
