Department of Biological Sciences and Pittsburgh NMR Center for Biomedical Research, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
NMR Biomed. 2013 Jul;26(7):860-71. doi: 10.1002/nbm.2948. Epub 2013 Apr 22.
This article presents a brief review of preclinical in vivo cell-tracking methods and applications using perfluorocarbon (PFC) probes and fluorine-19 ((19) F) MRI detection. Detection of the (19) F signal offers high cell specificity and quantification ability in spin density-weighted MR images. We discuss the compositions of matter, methods and applications of PFC-based cell tracking using ex vivo and in situ PFC labeling in preclinical studies of inflammation and cellular therapeutics. We also address the potential applicability of (19) F cell tracking to clinical trials.
本文简要回顾了使用全氟碳（PFC）探针和氟-19（19 F）MRI 检测进行临床前体内细胞示踪的方法和应用。（19 F）信号的检测在自旋密度加权磁共振图像中提供了高细胞特异性和定量能力。我们讨论了基于 PFC 的细胞示踪的物质组成、方法和应用，包括炎症和细胞治疗的临床前研究中的离体和原位 PFC 标记。我们还探讨了（19 F）细胞示踪在临床试验中的潜在适用性。
