Boyan L A, Bhargava G, Nishimura F, Orman R, Price R, Terranova V P
Laboratory of Tumor Biology and Connective Tissue Research, Columbia University, New York, New York 10032.
J Dent Res. 1994 Oct;73(10):1593-600. doi: 10.1177/00220345940730100301.
A major focus of studies that center on regeneration of the periodontium is to determine the efficacy of the use of polypeptide growth factors. Platelet-derived growth factor has been reported to be a possible agent for clinical use. PDGF has various isoforms. Therefore, we decided to study the mitogenic and chemotactic responses of human periodontal ligament (PDL) cells to recombinant human PDGF-AB, AA, and BB. Addition of each isoform of PDGF to in vitro mitogenesis assays induced PDL cell proliferation in a dose-dependent manner. The maximum mitogenic effect was evident at the concentration of 100 ng/mL. In these assays, PDGF-BB was found to be the most potent mitogen. PDGF-AB elicited an intermediate response, and PDGF-AA was the least effective. The results of chemotaxis assays closely parallel those of the mitogenesis assays. PDGF-BB exhibited the most potent chemotactic effect. The maximal effect was observed at 10 ng/mL. The findings of these experiments indicate that PDGF-BB is more effective than the other isoforms in promoting mitogenesis and chemotaxis of PDL cells in vitro, and may therefore be a suitable ethical pharmaceutical for use in periodontal regeneration procedures.
以牙周组织再生为中心的研究主要关注点是确定使用多肽生长因子的功效。据报道，血小板衍生生长因子是一种可能用于临床的药物。血小板衍生生长因子有多种亚型。因此，我们决定研究重组人血小板衍生生长因子-AB、AA和BB对人牙周膜细胞的促有丝分裂和趋化反应。在体外有丝分裂测定中添加每种血小板衍生生长因子亚型均以剂量依赖方式诱导牙周膜细胞增殖。在100 ng/mL浓度时，最大促有丝分裂效应明显。在这些测定中，发现血小板衍生生长因子-BB是最有效的促有丝分裂原。血小板衍生生长因子-AB引发中等反应，而血小板衍生生长因子-AA效果最差。趋化性测定结果与有丝分裂测定结果密切平行。血小板衍生生长因子-BB表现出最有效的趋化作用。在10 ng/mL时观察到最大效应。这些实验结果表明，在体外促进牙周膜细胞的有丝分裂和趋化作用方面，血小板衍生生长因子-BB比其他亚型更有效，因此可能是一种适用于牙周再生程序的符合伦理规范的药物。
