Ojima Y, Mizuno M, Kuboki Y, Komori T
Department of Oral and Maxillofacial Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Oral Dis. 2003 May;9(3):144-51. doi: 10.1034/j.1601-0825.2003.02906.x.
Platelet-derived growth factor (PDGF)-BB is a polypeptide growth factor which has been shown to stimulate periodontal regeneration. In this study, we investigated the time- and dose-dependent effect of PDGF-BB on the proliferation and collagen synthesis of human periodontal ligament (PDL) cells.
For the proliferation assay, PDL cells were cultured in 0.01-10 ng ml(-1) of PDGF-BB for 12 or 24 h, and cell numbers were counted. For the collagen synthesis assay, PDL cells were cultured in 0.1-10 ng ml(-1) of PDGF-BB for 1 to 24 h. The ratio of collagen content in total protein was evaluated, and the gene expression of type I collagen was assessed quantitatively by Northern blotting analysis.
PDGF-BB stimulated the proliferation of PDL cells in a time- and dose-dependent manner with the maximum effect at 10 ng ml(-1). PDGF-BB induced the collagen synthesis of PDL cells with the maximum effect for 24-h treatment, and 1 ng ml(-1) of PDGF-BB. PDGF-BB exhibits an inverse dose-dependent effect on proliferation and collagen synthesis by PDL cells. These findings suggest that PDGF-BB is one of the important regulators of the maintenance of the extracellular matrix in PDL, and may play an important role in the regeneration of PDL.
血小板衍生生长因子（PDGF）-BB是一种已被证明能刺激牙周组织再生的多肽生长因子。在本研究中，我们调查了PDGF-BB对人牙周膜（PDL）细胞增殖和胶原合成的时间和剂量依赖性影响。
在增殖试验中，将PDL细胞培养于0.01-10 ng/ml的PDGF-BB中12或24小时，然后计数细胞数量。在胶原合成试验中，将PDL细胞培养于0.1-10 ng/ml的PDGF-BB中1至24小时。评估总蛋白中胶原含量的比例，并通过Northern印迹分析定量评估I型胶原的基因表达。
PDGF-BB以时间和剂量依赖性方式刺激PDL细胞增殖，在10 ng/ml时效果最佳。PDGF-BB诱导PDL细胞的胶原合成，24小时处理且浓度为1 ng/ml时效果最佳。PDGF-BB对PDL细胞的增殖和胶原合成表现出相反的剂量依赖性作用。这些发现表明，PDGF-BB是PDL中细胞外基质维持的重要调节因子之一，可能在PDL的再生中发挥重要作用。
