Kool J, De Visser H, Gerrits-Boeye M Y, Klasen I S, Melief M J, Van Helden-Meeuwsen C G, Van Lieshout L M, Ruseler-Van Embden J G, Van den Berg W B, Bahr G M
Department of Immunology, Erasmus University Rotterdam, The Netherlands.
J Histochem Cytochem. 1994 Nov;42(11):1435-41. doi: 10.1177/42.11.7930525.
We studied the presence of bacterial antigens in rat tissues. We produced a monoclonal antibody (MAb 2E9) directed against intestinal flora-derived peptidoglycan-polysaccharide complexes from human and rat feces. With several immunological techniques, the specificity of 2E9 for this bacterial product was demonstrated. Using 2E9 in an immunohistological assay, we were able to show the presence of bacterial products in macrophages in the red pulp of spleens of conventional Lewis rats. However, we found no correlation between the development of the intestinal flora and positive spleen staining with MAb 2E9. The results were confirmed by immunohistology with a previously described MAb 2-4 directed to muramyl dipeptide. Other lymphoid organs did not stain positively with 2E9 and 2-4. Neonatal and young rats showed no staining of the spleen, but positivity could be induced by injecting peptidoglycan-polysaccharide complexes systemically. We conclude that bacterial fragments are present in splenic macrophages of conventional rats.
我们研究了大鼠组织中细菌抗原的存在情况。我们制备了一种单克隆抗体（MAb 2E9），它针对来自人和大鼠粪便的肠道菌群衍生的肽聚糖 - 多糖复合物。通过几种免疫学技术，证明了2E9对这种细菌产物的特异性。在免疫组织学检测中使用2E9，我们能够在常规Lewis大鼠脾脏红髓中的巨噬细胞中显示细菌产物的存在。然而，我们发现肠道菌群的发育与MAb 2E9对脾脏的阳性染色之间没有相关性。用先前描述的针对胞壁酰二肽的MAb 2 - 4进行免疫组织学证实了结果。其他淋巴器官用2E9和2 - 4染色未呈阳性。新生大鼠和幼鼠的脾脏未染色，但通过全身注射肽聚糖 - 多糖复合物可诱导阳性。我们得出结论，常规大鼠的脾巨噬细胞中存在细菌片段。
