Alrefai R H, Shiel P J, Domier L L, D'Arcy C J, Berger P H, Korban S S
University of Illinois, Department of Horticulture, Plant and Animal Biotechnology, Urbana 61801.
J Gen Virol. 1994 Oct;75 ( Pt 10):2847-50. doi: 10.1099/0022-1317-75-10-2847.
A double-stranded cDNA was synthesized from in vitro polyadenylated apple mosaic virus (ApMV) RNA 3 using oligo(dT) and sequence-specific primers, and was cloned into plasmid vectors. A set of overlapping cDNA clones was used to determine the nucleotide sequence of RNA 4. ApMV RNA 4 was found to contain an open reading frame (ORF) of 666 nucleotides, which was flanked by 5' and 3' non-translated sequences of 55 and 264 nucleotides, respectively. The ORF encoding the coat protein was identified by comparing the predicted amino acid sequence with that obtained from direct protein microsequencing of the native viral coat protein. The ORF encodes a protein with an M(r) of 25,056. The nucleotide sequence of the ApMV coat protein gene showed no similarity to those of alfalfa mosaic virus, tobacco streak virus (TSV), brome mosaic virus or cucumber mosaic virus. The predicted amino acid sequence of the amino-terminal region of the ApMV coat protein is basic, rich in cysteine residues and may contain a zinc finger motif similar to that found in TSV.
使用寡聚（dT）和序列特异性引物，从体外聚腺苷酸化的苹果花叶病毒（ApMV）RNA 3合成双链cDNA，并将其克隆到质粒载体中。一组重叠的cDNA克隆用于确定RNA 4的核苷酸序列。发现ApMV RNA 4含有一个666个核苷酸的开放阅读框（ORF），其两侧分别是55个和264个核苷酸的5'和3'非翻译序列。通过将预测的氨基酸序列与从天然病毒外壳蛋白的直接蛋白质微量测序获得的序列进行比较，鉴定了编码外壳蛋白的ORF。该ORF编码一种分子量为25,056的蛋白质。ApMV外壳蛋白基因的核苷酸序列与苜蓿花叶病毒、烟草线条病毒（TSV）、雀麦花叶病毒或黄瓜花叶病毒的核苷酸序列没有相似性。ApMV外壳蛋白氨基末端区域的预测氨基酸序列呈碱性，富含半胱氨酸残基，可能含有与TSV中发现的类似的锌指基序。
