Ge X, Scott S W
Department of Plant Pathology and Physiology, Clemson University, SC 29634-0377, USA.
Virus Res. 1996 Jan;40(1):57-63. doi: 10.1016/0168-1702(95)01251-6.
The complete nucleotide sequence of the RNA 3 of hydrangea mosaic virus (HdMV) was determined. It consists of 2268 nucleotides and contains two open reading frames (ORF). ORF 1 encodes for a putative translation product of 293 amino acids which shared 64.9% identity with the 3a protein of tobacco streak virus (TSV). ORF 2 encodes for a putative translation product of 220 amino acids which shared 54.2% identity with the coat protein of TSV. The relationship between the proteins of HdMV and the corresponding proteins of ilarviruses other than TSV was more distant. No zinc-finger-like motif was found in the coat protein of HdMV but the N-terminus of the protein was rich in basic amino acids. Both terminal, non-coding regions of HdMV RNA 3 contained repeated sequences with corresponding homologous fragments in the RNA 3 of TSV. On the basis of the similarities between HdMV and TSV that we detected, we propose that HdMV be included in subgroup 1 of the genus Ilarvirus together with TSV.
测定了绣球花叶病毒（HdMV）RNA 3的完整核苷酸序列。它由2268个核苷酸组成，包含两个开放阅读框（ORF）。ORF 1编码一个推定的293个氨基酸的翻译产物，该产物与烟草线条病毒（TSV）的3a蛋白具有64.9%的同一性。ORF 2编码一个推定的220个氨基酸的翻译产物，该产物与TSV的外壳蛋白具有54.2%的同一性。HdMV的蛋白质与除TSV之外的其他等轴不稳环斑病毒属病毒的相应蛋白质之间的关系更为疏远。在HdMV的外壳蛋白中未发现锌指样基序，但该蛋白的N端富含碱性氨基酸。HdMV RNA 3的两个末端非编码区都含有重复序列，在TSV的RNA 3中具有相应的同源片段。基于我们检测到的HdMV和TSV之间的相似性，我们建议将HdMV与TSV一起归入等轴不稳环斑病毒属的第1亚组。
