Dudley M A, Wang H, Hachey D L, Shulman R J, Perkinson J S, Rosenberger J, Mersmann H J
USDA/ARS Children's Nutrition Research Center, Baylor College of Medicine, Department of Pediatrics, Houston, TX 77030.
J Nutr. 1994 Oct;124(10):1996-2005. doi: 10.1093/jn/124.10.1996.
We tested the effect of dietary fat on the lipid composition and hydrolase activity of jejunal brush border membranes in piglets. Eighteen 5-wk-old piglets were divided into three groups and for 4 wk fed either an unsaturated low fat diet (3.2% corn oil), an unsaturated high fat diet (17.2% corn oil) or a saturated high fat diet (2.2% corn oil + 15% tallow). Brush border membranes were prepared from the jejunal mucosa and analyzed for cholesterol, phospholipid and fatty acids. The activities of sucrase-isomaltase, lactase-phlorizin hydrolase, maltase-glucoamylase, aminopeptidase and alkaline phosphatase were measured. Lactase-phlorizin hydrolase isoforms were immunopurified and separated by SDS-PAGE, and their relative proportions were measured by densitometry. The activities of the disaccharidases and alkaline phosphatase, but not aminopeptidase, were greater in animals fed the saturated high fat diet than in animals fed the unsaturated high fat diet. The fatty acid composition of the membranes generally reflected the composition of the diet. Correlation analysis demonstrated that the phospholipid, fatty acid and cholesterol compositions of the membranes were associated with the differences in brush border hydrolase activity.
我们检测了日粮脂肪对仔猪空肠刷状缘膜脂质组成和水解酶活性的影响。将18头5周龄仔猪分为三组，分别饲喂不饱和低脂日粮（3.2%玉米油）、不饱和高脂日粮（17.2%玉米油）或饱和高脂日粮（2.2%玉米油+15%牛脂）4周。从空肠黏膜制备刷状缘膜，并分析其胆固醇、磷脂和脂肪酸含量。测定蔗糖酶-异麦芽糖酶、乳糖酶-根皮苷水解酶、麦芽糖酶-葡糖淀粉酶、氨肽酶和碱性磷酸酶的活性。对乳糖酶-根皮苷水解酶同工型进行免疫纯化，通过SDS-PAGE分离，并通过光密度法测定其相对比例。与饲喂不饱和高脂日粮的动物相比，饲喂饱和高脂日粮的动物二糖酶和碱性磷酸酶的活性更高，但氨肽酶活性并非如此。膜的脂肪酸组成总体上反映了日粮的组成。相关性分析表明，膜的磷脂、脂肪酸和胆固醇组成与刷状缘水解酶活性的差异相关。
