在特定条件下通过辐照杀灭不同株系的刚地弓形虫组织包囊
Killing of different strains of Toxoplasma gondii tissue cysts by irradiation under defined conditions.
作者信息
Dubey J P, Thayer D W
机构信息
Parasite Biology and Epidemiology Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705-2350.
出版信息
J Parasitol. 1994 Oct;80(5):764-7.
To study the effect of gamma-irradiation on the viability of Toxoplasma gondii tissue cysts, brains of mice inoculated with 95 newly isolated strains of T. gondii from pigs and 10 other laboratory isolates were pooled, flattened, packed in vacuum, and irradiated to absorbed doses of 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, and 0.9 kGy at 5 C (+/- 0.5 C) with a 137Cs gamma-radiation source. Treated samples were bioassayed for viable T. gondii in mice, cats, or both. Tissue cysts of all strains were rendered nonviable at 0.4 kGy. To study the effect of temperature on inactivation by irradiation, tissue cysts were irradiated at -4, 0, 4, 8, 12, and 16 C (+/- 0.5 C) at 0.25 kGy. Temperature during irradiation had no marked effect on the viability of tissue cysts.
为研究γ射线辐照对刚地弓形虫组织包囊活力的影响，将接种了95株新分离自猪的刚地弓形虫菌株和10株其他实验室分离株的小鼠大脑汇集、铺平、真空包装，并用137Csγ射线源在5℃（±0.5℃）下辐照至吸收剂量为0.1、0.2、0.3、0.4、0.5、0.6、0.7和0.9千戈瑞。对处理后的样本在小鼠、猫或两者身上进行活的刚地弓形虫的生物测定。所有菌株的组织包囊在0.4千戈瑞时均失去活力。为研究温度对辐照灭活的影响，将组织包囊在-4、0、4、8、12和16℃（±0.5℃）下以0.25千戈瑞进行辐照。辐照期间的温度对组织包囊的活力没有显著影响。
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