Hassanen Eman A A, Makau Dennis N, Afifi Mohamed, Al-Jabr Omar A, Abdulrahman Alshahrani Mohammed, Saif Ahmed, Anter Reham G A, El-Neshwy Wafaa M, Ibrahim Doaa, Abou Elez Rasha M M
Department of Parasitology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN, United States.
Front Vet Sci. 2023 Mar 24;10:1147614. doi: 10.3389/fvets.2023.1147614. eCollection 2023.
Toxoplasmosis is a global zoonotic disease caused by (). The primary aim of this study was to identify hygienic and cat management practices that could affect the occurrence of in cats and their owners in Sharqia Governorate, Egypt. infection was evaluated in 80 pregnant women and 29 domestic cats using Real-time PCR. A questionnaire was administered to obtain information regarding the risk factors associated with infection. Blood samples were collected from enrolled pregnant women, and fecal samples were collected from their pet cats. Firth logistic regression model complemented with random forest (RF) analysis was used to evaluate the association of different hygiene and cat management practices with infection in pregnant women. infection was identified in 27.5% of pregnant women and 17% of domestic cats. Drinking raw milk and contacting stray and pet cats were significantly associated with higher odds of infection. The proportion of -positive women who ate raw meat (60.0%) was higher than those ate heat-cooked meat (25.3%). Moreover, women who did not wash their hands after contact with cats were 6 times (OR 6.12; CI: 3.03-9.21) more likely to experience infection than those washed their hands after cat contact. The RF analysis showed that washing hands constitute a serious yet preventable public health concern that requires targeted, community-specific efforts. Cat owners, particularly pregnant women, need to be aware about the risk of infection, while handling cat and pet's owner should be advised to take necessary hygienic measures to prevent its infection.
弓形虫病是一种由（）引起的全球性人畜共患病。本研究的主要目的是确定可能影响埃及谢赫村省猫及其主人感染（）情况的卫生和猫管理措施。采用实时荧光定量聚合酶链反应（Real-time PCR）对80名孕妇和29只家猫进行了（）感染评估。通过问卷调查获取与（）感染相关的危险因素信息。采集了纳入研究的孕妇的血液样本，并采集了她们宠物猫的粪便样本。使用基于费思逻辑回归模型并辅以随机森林（RF）分析来评估不同卫生和猫管理措施与孕妇（）感染之间的关联。在27.5%的孕妇和17%的家猫中检测到（）感染。饮用生牛奶以及接触流浪猫和宠物猫与（）感染几率较高显著相关。食用生肉的（）阳性女性比例（60.0%）高于食用经加热烹制肉类的女性（25.3%）。此外，接触猫后不洗手的女性感染（）的可能性是接触猫后洗手女性的6倍（比值比6.12；置信区间：3.03 - 9.21）。随机森林分析表明，洗手是一个严重但可预防的公共卫生问题，需要针对性地、针对特定社区开展工作。猫主人，尤其是孕妇，需要意识到（）感染的风险，同时在处理猫时，应建议宠物主人采取必要的卫生措施以预防感染。
