Fujita T, Sakuma S, Takahashi K, Bohtani Y, Nishida H, Fujimoto Y
Department of Hygienic Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
Res Commun Mol Pathol Pharmacol. 1997 May;96(2):203-8.
The effects of Fe2+, Cu2+, Zn2+ and Se4+ on the activity of glucosamine synthetase, the rate-limiting enzyme of mucus synthesis, in rabbit gastric corporal mucosa were examined. Cu2+, Zn2+ and Se4+ inhibited the glucosamine synthetase activity at concentrations ranging from 1 to 10 microM (Cu2+, 8-98% inhibition; Zn2+, 10-99% inhibition; Se4+, 32-89% inhibition). The inhibitory effects of these three ions were much stronger than that of UDP-N-acetylglúcosamine known as a representative inhibitor of the glucosamine synthetase activity (10 microM, 52% inhibition). Fe2+ had no significant effect on the glucosamine synthetase activity up to 100 microM. These results suggest that Cu2+, Zn2+ and Se4+ can be potent inhibitors of gastric glucosamine synthetase activity.
研究了Fe2+、Cu2+、Zn2+和Se4+对兔胃体黏膜中黏液合成限速酶氨基葡萄糖合成酶活性的影响。Cu2+、Zn2+和Se4+在1至10微摩尔的浓度范围内抑制氨基葡萄糖合成酶活性（Cu2+，抑制率为8 - 98%；Zn2+，抑制率为10 - 99%；Se4+，抑制率为32 - 89%）。这三种离子的抑制作用比已知的氨基葡萄糖合成酶活性代表性抑制剂UDP - N - 乙酰葡糖胺（10微摩尔，抑制率52%）要强得多。高达100微摩尔时，Fe2+对氨基葡萄糖合成酶活性无显著影响。这些结果表明，Cu2+、Zn2+和Se4+可能是胃氨基葡萄糖合成酶活性的有效抑制剂。
