Countryman J K, Heston L, Gradoville L, Himmelfarb H, Serdy S, Miller G
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06510.
J Virol. 1994 Nov;68(11):7628-33. doi: 10.1128/JVI.68.11.7628-7633.1994.
ZEBRA has been shown to activate model reporter genes consisting of synthetic oligomerized ZEBRA response elements upstream of a minimal CYC1 promoter fused to beta-galactosidase in the yeast Saccharomyces cerevisiae. Here it is shown that in S. cerevisiae ZEBRA activates transcription of natural Epstein-Barr virus promoters. Two Epstein-Barr virus promoters were shown to be activated by ZEBRA in S. cerevisiae: Zp, the promoter that regulates expression of BZLF1, which encodes ZEBRA; and EAp, the promoter controlling expression of BMRF1, which encodes diffuse early antigen, EA-D. These observations indicate that neither mammalian-specific nor virally encoded coactivators are obligatory for ZEBRA to stimulate expression from these two promoters. Zp was also strongly activated by endogenous yeast factors. EAp was not activated by yeast factors. The results show that in S. cerevisiae and in B cells, ZEBRA dominates the response of EAp; ZEBRA plus endogenous cell factors activate Zp.
在酿酒酵母中，ZEBRA已被证明可激活模型报告基因，该基因由最小CYC1启动子上游的合成寡聚化ZEBRA反应元件组成，该启动子与β-半乳糖苷酶融合。本文表明，在酿酒酵母中，ZEBRA可激活天然爱泼斯坦-巴尔病毒启动子的转录。在酿酒酵母中，两个爱泼斯坦-巴尔病毒启动子被证明可被ZEBRA激活：Zp，即调节编码ZEBRA的BZLF1表达的启动子；以及EAp，即控制编码弥散早期抗原EA-D的BMRF1表达的启动子。这些观察结果表明，对于ZEBRA刺激这两个启动子的表达而言，既不需要哺乳动物特异性的共激活因子，也不需要病毒编码的共激活因子。Zp也被内源性酵母因子强烈激活。EAp未被酵母因子激活。结果表明，在酿酒酵母和B细胞中，ZEBRA主导了EAp的反应；ZEBRA加上内源性细胞因子可激活Zp。
