• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

新城疫病毒融合蛋白的融合肽和七肽重复区域中的突变会阻断融合。

Mutations in the fusion peptide and heptad repeat regions of the Newcastle disease virus fusion protein block fusion.

作者信息

Sergel-Germano T, McQuain C, Morrison T

机构信息

Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655.

出版信息

J Virol. 1994 Nov;68(11):7654-8. doi: 10.1128/JVI.68.11.7654-7658.1994.

DOI:10.1128/JVI.68.11.7654-7658.1994
PMID:7933158
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC237219/
Abstract

Nonconservative mutations were introduced by site-specific mutagenesis into the fusion peptide and the adjacent heptad repeat region of the fusion protein of Newcastle disease virus in order to determine the role of both regions in the fusion activity of the protein. Mutations in both regions that allowed for proper folding and intracellular transport of the protein blocked the fusion activity of the protein when assayed in the presence of the hemagglutinin-neuraminidase protein.

摘要

通过定点诱变将非保守突变引入新城疫病毒融合蛋白的融合肽和相邻的七肽重复区域，以确定这两个区域在该蛋白融合活性中的作用。在血凝素神经氨酸酶蛋白存在的情况下进行检测时，这两个区域中允许蛋白正确折叠和细胞内运输的突变会阻断该蛋白的融合活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0884/237219/a543f0089bad/jvirol00020-0845-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0884/237219/0022031e5a80/jvirol00020-0844-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0884/237219/a807bf527356/jvirol00020-0844-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0884/237219/a543f0089bad/jvirol00020-0845-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0884/237219/0022031e5a80/jvirol00020-0844-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0884/237219/a807bf527356/jvirol00020-0844-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0884/237219/a543f0089bad/jvirol00020-0845-a.jpg

相似文献

1
Mutations in the fusion peptide and heptad repeat regions of the Newcastle disease virus fusion protein block fusion.新城疫病毒融合蛋白的融合肽和七肽重复区域中的突变会阻断融合。
J Virol. 1994 Nov;68(11):7654-8. doi: 10.1128/JVI.68.11.7654-7658.1994.
2
'a'-Position-mutated and G4-mutated hemagglutinin-neuraminidase proteins of Newcastle disease virus impair fusion and hemagglutinin-neuraminidase-fusion interaction by different mechanisms.a-位置突变和 G4 突变的新城疫病毒血凝素-神经氨酸酶蛋白通过不同的机制损害融合和血凝素-神经氨酸酶-融合相互作用。
Intervirology. 2013;56(1):27-36. doi: 10.1159/000341613. Epub 2012 Oct 4.
3
Mutations in the fusion peptide and adjacent heptad repeat inhibit folding or activity of the Newcastle disease virus fusion protein.融合肽和相邻七肽重复序列中的突变会抑制新城疫病毒融合蛋白的折叠或活性。
J Virol. 2001 Sep;75(17):7934-43. doi: 10.1128/jvi.75.17.7934-7943.2001.
4
Interacting domains of the HN and F proteins of newcastle disease virus.新城疫病毒HN蛋白与F蛋白的相互作用结构域
J Virol. 2003 Oct;77(20):11040-9. doi: 10.1128/jvi.77.20.11040-11049.2003.
5
The role of leucine residues in the structure and function of a leucine zipper peptide inhibitor of paramyxovirus (NDV) fusion.
Virology. 1998 Mar 30;243(1):21-31. doi: 10.1006/viro.1998.9044.
6
Amino acid substitutions in the F-specific domain in the stalk of the newcastle disease virus HN protein modulate fusion and interfere with its interaction with the F protein.新城疫病毒HN蛋白柄部F特异性结构域中的氨基酸取代可调节融合并干扰其与F蛋白的相互作用。
J Virol. 2004 Dec;78(23):13053-61. doi: 10.1128/JVI.78.23.13053-13061.2004.
7
Six-helix bundle assembly and characterization of heptad repeat regions from the F protein of Newcastle disease virus.新城疫病毒F蛋白七肽重复区域的六螺旋束组装及特性分析
J Gen Virol. 2002 Mar;83(Pt 3):623-629. doi: 10.1099/0022-1317-83-3-623.
8
A single amino acid change in the Newcastle disease virus fusion protein alters the requirement for HN protein in fusion.新城疫病毒融合蛋白中的单个氨基酸变化改变了融合过程中对血凝素神经氨酸酶蛋白的需求。
J Virol. 2000 Jun;74(11):5101-7. doi: 10.1128/jvi.74.11.5101-5107.2000.
9
Direct evidence that the N-terminal heptad repeat of Sendai virus fusion protein participates in membrane fusion.仙台病毒融合蛋白N端七肽重复序列参与膜融合的直接证据。
J Mol Biol. 1999 Sep 24;292(3):531-46. doi: 10.1006/jmbi.1999.3097.
10
Mutational analysis of the membrane proximal heptad repeat of the newcastle disease virus fusion protein.新城疫病毒融合蛋白膜近端七肽重复序列的突变分析
Virology. 2001 Oct 25;289(2):343-52. doi: 10.1006/viro.2001.1123.

引用本文的文献

1
Effects of serial passaging of field isolates of Bangladeshi PPR virus in Vero cells on the fusion protein.孟加拉国小反刍兽疫病毒野外分离株在Vero细胞中连续传代对融合蛋白的影响
Ir Vet J. 2025 Jul 1;78(1):14. doi: 10.1186/s13620-025-00298-z.
2
Phylogeny and Pathogenicity of Subtype XIIb NDVs from Francolins in Southwestern China and Effective Protection by an Inactivated Vaccine.中国西南部鹧鸪源XIIb亚型新城疫病毒的系统发育与致病性及灭活疫苗的有效保护作用
Transbound Emerg Dis. 2023 Apr 5;2023:1317784. doi: 10.1155/2023/1317784. eCollection 2023.
3
Analysis of Amino Acid Changes in the Fusion Protein of Virulent Newcastle Disease Virus from Vaccinated Poultry in Nigerian Isolates.

本文引用的文献

1
A spring-loaded mechanism for the conformational change of influenza hemagglutinin.一种用于流感血凝素构象变化的弹簧加载机制。
Cell. 1993 May 21;73(4):823-32. doi: 10.1016/0092-8674(93)90260-w.
2
The attachment function of the Newcastle disease virus hemagglutinin-neuraminidase protein can be separated from fusion promotion by mutation.新城疫病毒血凝素-神经氨酸酶蛋白的附着功能可通过突变与融合促进作用分离。
Virology. 1993 Apr;193(2):717-26. doi: 10.1006/viro.1993.1180.
3
Mutations in the transmembrane domain of the HN protein of Newcastle disease virus affect the structure and activity of the protein.
尼日利亚分离株中接种疫苗家禽的强毒新城疫病毒融合蛋白氨基酸变化分析
Int J Microbiol. 2022 Oct 31;2022:9979683. doi: 10.1155/2022/9979683. eCollection 2022.
4
Avian Type 1 in Egypt: Epidemiology, Evolutionary Perspective, and Vaccine Approach.埃及的禽1型：流行病学、进化视角及疫苗方法
Front Vet Sci. 2021 Jul 15;8:647462. doi: 10.3389/fvets.2021.647462. eCollection 2021.
5
Peptide and peptide-based inhibitors of SARS-CoV-2 entry.SARS-CoV-2 进入抑制剂的肽和基于肽的抑制剂。
Adv Drug Deliv Rev. 2020 Dec;167:47-65. doi: 10.1016/j.addr.2020.11.007. Epub 2020 Nov 13.
6
Insights into Genomic Epidemiology, Evolution, and Transmission Dynamics of Genotype VII of Class II Newcastle Disease Virus in China.中国II类新城疫病毒VII基因型的基因组流行病学、进化及传播动力学研究
Pathogens. 2020 Oct 13;9(10):837. doi: 10.3390/pathogens9100837.
7
Sequence analysis and pathogenicity of Avian Orthoavulavirus 1 strains isolated from poultry flocks during 2015-2019.2015-2019 年期间从禽群中分离的禽正黏病毒 1 株的序列分析及致病性。
BMC Vet Res. 2020 Jul 22;16(1):253. doi: 10.1186/s12917-020-02470-9.
8
Molecular characterization of full genome sequences of Newcastle disease viruses circulating among vaccinated chickens in Egypt during 2011-2013.2011-2013 年埃及接种疫苗鸡群中流行的新城疫病毒全基因组序列的分子特征。
J Vet Med Sci. 2020 Jun 24;82(6):809-816. doi: 10.1292/jvms.19-0623. Epub 2020 Apr 20.
9
The DI-DII linker of human parainfluenza virus type 3 fusion protein is critical for the virus.人副流感病毒3型融合蛋白的DI-DII连接区对该病毒至关重要。
Virus Genes. 2020 Feb;56(1):37-48. doi: 10.1007/s11262-019-01713-8. Epub 2019 Nov 25.
10
Two mutations in the HR2 region of Newcastle disease virus fusion protein with a cleavage motif "RRQRRL" are critical for fusogenic activity.新城疫病毒融合蛋白 HR2 区的两个具有“RRQRRL”切割基序的突变对于融合活性至关重要。
Virol J. 2017 Sep 25;14(1):185. doi: 10.1186/s12985-017-0851-0.
新城疫病毒HN蛋白跨膜结构域的突变会影响该蛋白的结构和活性。
Virology. 1993 Sep;196(1):101-10. doi: 10.1006/viro.1993.1458.
4
Paramyxovirus fusion: a hypothesis for changes.副粘病毒融合：变化的一种假说。
Virology. 1993 Nov;197(1):1-11. doi: 10.1006/viro.1993.1561.
5
The role of the individual cysteine residues in the formation of the mature, antigenic HN protein of Newcastle disease virus.单个半胱氨酸残基在新城疫病毒成熟抗原性HN蛋白形成中的作用。
Virology. 1994 May 1;200(2):470-83. doi: 10.1006/viro.1994.1210.
6
Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus.副粘病毒糖蛋白生物活性的鉴定。仙台病毒无活性前体蛋白经蛋白水解切割后细胞融合、溶血及感染性的激活。
Virology. 1974 Feb;57(2):475-90. doi: 10.1016/0042-6822(74)90187-1.
7
Purification and amino-terminal protein sequence analysis of the mumps virus fusion protein.腮腺炎病毒融合蛋白的纯化及氨基末端蛋白序列分析
Virology. 1985 Jul 30;144(2):373-83. doi: 10.1016/0042-6822(85)90279-x.
8
Conformational changes in Newcastle disease virus fusion glycoprotein during intracellular transport.新城疫病毒融合糖蛋白在细胞内运输过程中的构象变化。
J Virol. 1985 Nov;56(2):341-8. doi: 10.1128/JVI.56.2.341-348.1985.
9
Intracellular processing of the Newcastle disease virus fusion glycoprotein.新城疫病毒融合糖蛋白的细胞内加工过程。
J Virol. 1985 Mar;53(3):851-7. doi: 10.1128/JVI.53.3.851-857.1985.
10
The nucleotide sequence of the mRNA encoding the fusion protein of measles virus (Edmonston strain): a comparison of fusion proteins from several different paramyxoviruses.编码麻疹病毒（埃德蒙斯顿株）融合蛋白的mRNA的核苷酸序列：几种不同副粘病毒融合蛋白的比较。
Virology. 1986 Dec;155(2):508-23. doi: 10.1016/0042-6822(86)90212-6.