新城疫病毒融合蛋白的融合肽和七肽重复区域中的突变会阻断融合。
Mutations in the fusion peptide and heptad repeat regions of the Newcastle disease virus fusion protein block fusion.
作者信息
Sergel-Germano T, McQuain C, Morrison T
机构信息
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655.
出版信息
J Virol. 1994 Nov;68(11):7654-8. doi: 10.1128/JVI.68.11.7654-7658.1994.
Abstract
Nonconservative mutations were introduced by site-specific mutagenesis into the fusion peptide and the adjacent heptad repeat region of the fusion protein of Newcastle disease virus in order to determine the role of both regions in the fusion activity of the protein. Mutations in both regions that allowed for proper folding and intracellular transport of the protein blocked the fusion activity of the protein when assayed in the presence of the hemagglutinin-neuraminidase protein.
摘要
通过定点诱变将非保守突变引入新城疫病毒融合蛋白的融合肽和相邻的七肽重复区域，以确定这两个区域在该蛋白融合活性中的作用。在血凝素神经氨酸酶蛋白存在的情况下进行检测时，这两个区域中允许蛋白正确折叠和细胞内运输的突变会阻断该蛋白的融合活性。
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