Murphy M F, Klieforth R B, Lahijani R S, Heuschele W P
Salk Institute Biotechnology/Industrial Associates, Inc., La Jolla, California 92037.
J Wildl Dis. 1994 Jul;30(3):377-82. doi: 10.7589/0090-3558-30.3.377.
We derived sequence information from cloned HindIII fragment "D" of alcelaphine herpesvirus 1 strain WC11, an agent of malignant catarrhal fever (MCF). Based on this sequence, oligonucleotide primers were selected and synthesized for use in a polymerase chain reaction amplification assay. These primers were used to test samples of total nucleic acids isolated from multiple tissues taken from an Indian gaur (Bos guarus gaurus) at the San Diego Wild Animal Park in San Diego, California (USA) which had clinical signs of a natural infection of MCF. Six of eight tissue samples examined had amplifiable sequences present. A nucleic acid probe complementary to the sequence of the original clone between the primer sites also was synthesized and used to confirm the identity of the amplified viral sequences, thus providing a diagnosis of MCF at the molecular level.
我们从恶性卡他热（MCF）病原体——非洲马瘟病毒1型WC11株的克隆HindIII片段“D”中获得了序列信息。基于该序列，选择并合成了寡核苷酸引物，用于聚合酶链反应扩增检测。这些引物用于检测从美国加利福尼亚州圣地亚哥野生动物公园的一头印度野牛（Bos guarus gaurus）采集的多个组织中分离出的总核酸样本，该野牛有MCF自然感染的临床症状。所检测的八个组织样本中有六个存在可扩增序列。还合成了与引物位点之间原始克隆序列互补的核酸探针，并用于确认扩增病毒序列的身份，从而在分子水平上提供了MCF的诊断。
