Michel A L
Department of Virology, Onderstepoort Veterinary Institute, South Africa.
Onderstepoort J Vet Res. 1993 Jun;60(2):87-93.
Two WC 11 specific DNA fragments, 3 kb and 2 kb in size, respectively, were cloned and evaluated as probes for their use in diagnostic and epidemiological investigations of malignant catarrhal fever (MCF). Field specimens including blood, ocular fluid, nasal mucus and urine of blue wildebeest (Connochaetes taurinus) calves in the Kruger National Park, South Africa, were tested and found positive for excretion of MCF-virus by slot blot hybridization. In 2 cases MCF-virus DNA was detected in the urine of the calves. No hybridization was observed with DNA from other bovine herpesviruses.
分别克隆了两个大小为3 kb和2 kb的WC 11特异性DNA片段，并对其作为探针用于恶性卡他热（MCF）诊断和流行病学调查的用途进行了评估。对南非克鲁格国家公园蓝角马（Connochaetes taurinus）犊牛的血液、眼液、鼻黏液和尿液等野外样本进行了检测，通过狭缝印迹杂交发现这些样本中MCF病毒排泄呈阳性。在2例中，在犊牛尿液中检测到了MCF病毒DNA。未观察到与其他牛疱疹病毒DNA的杂交。
