Kees-Folts D, Sadow J L, Schreiner G F
Department of Pediatrics, Hershey Medical Center, Pennsylvania.
Kidney Int. 1994 Jun;45(6):1697-709. doi: 10.1038/ki.1994.222.
Proteinuria and tubulointerstitial inflammation (TII) correlate with progression to renal failure in human glomerulonephritis. Various forms of experimental nephrotic syndrome are associated with TII. To study the genesis of TII, we utilized the model of albumin overload. Rats received intraperitoneal bovine serum albumin (BSA) for 1 to 14 days, developing heavy proteinuria. A predominantly macrophage interstitial infiltrate was present at days 3, 7 and 14. The urine of the rats contained a factor chemotactic for macrophages which partitioned into the organic phase with ethyl acetate extraction. TLC and HPLC characteristics were those of a novel, non-polar lipid. Supernatant from the culture of proximal tubule (PT) segments after in vivo or in vitro exposure to high concentrations of lipid-replete BSA showed chemotactic activity with similar chromatographic characteristics. PT cultured with delipidated BSA produced little activity. Thus, the generation of this inflammatory factor occurs as a consequence of tubular metabolism of albumin-borne fatty acids and may contribute to the development of proteinuria-associated TII.
蛋白尿和肾小管间质炎症（TII）与人类肾小球肾炎进展至肾衰竭相关。多种形式的实验性肾病综合征都与TII有关。为了研究TII的发生机制，我们采用了白蛋白超负荷模型。大鼠腹腔注射牛血清白蛋白（BSA）1至14天，出现大量蛋白尿。在第3天、第7天和第14天，主要有巨噬细胞间质浸润。大鼠尿液中含有一种对巨噬细胞有趋化作用的因子，用乙酸乙酯萃取后可分配到有机相中。薄层色谱（TLC）和高效液相色谱（HPLC）特征表明这是一种新型的非极性脂质。体内或体外暴露于高浓度富含脂质的BSA后，近端肾小管（PT）节段培养物的上清液表现出具有相似色谱特征的趋化活性。用脱脂BSA培养的PT产生的活性很小。因此，这种炎性因子的产生是白蛋白携带的脂肪酸经肾小管代谢的结果，可能有助于蛋白尿相关TII的发展。
