Eisenhauer C L, Matsuda L S, Uyehara C F
Department of Clinical Investigation, Tripler Army Medical Center, Tripler AMC, HI 96859-5000.
Lab Anim Sci. 1994 Jun;44(3):245-52.
The newborn piglet is becoming increasingly popular as a model for neonatal studies. However, data on normal physiologic baseline values and the influence of anesthesia on these values are scarce. In this study, we describe husbandry, surgical, and experimental methods used to establish a conscious, chronically catheterized neonatal piglet model, which enabled study of unrestrained piglets recovered from surgery and anesthesia, for up to 10 days after separation from the sow. Chronic catheterization allowed repeated experiments to be performed in the same animals, reducing the number of animals needed for study. Presented herein are baseline resting hemodynamic and blood chemistry data and circulating hormone measurements of vasopressin, plasma renin activity, adrenocorticotrophic hormone, cortisol, epinephrine, norepinephrine, and dopamine for piglets in the first 2 weeks of life. Also, in two series of experiments, the effects of the gas anesthetic isoflurane and the injectable anesthetic pentobarbital on these baseline values were investigated. Results indicate that both pentobarbital and isoflurane elicit changes in blood pressure, heart rate, vasopressin, plasma renin activity and ventilatory drive that should be considered when using either of these anesthetic agents in acute studies.
新生仔猪作为新生儿研究的模型正变得越来越受欢迎。然而，关于正常生理基线值以及麻醉对这些值的影响的数据却很稀少。在本研究中，我们描述了用于建立清醒、长期插管的新生仔猪模型的饲养、手术和实验方法，该模型能够研究从手术和麻醉中恢复的未受约束的仔猪，在与母猪分离后的长达10天内的情况。长期插管允许在同一动物身上重复进行实验，从而减少了研究所需的动物数量。本文给出了新生仔猪出生后前两周的静息血流动力学和血液化学基线数据，以及血管加压素、血浆肾素活性、促肾上腺皮质激素、皮质醇、肾上腺素、去甲肾上腺素和多巴胺的循环激素测量值。此外，在两个系列的实验中，研究了气体麻醉剂异氟烷和注射用麻醉剂戊巴比妥对这些基线值的影响。结果表明，在急性研究中使用这两种麻醉剂中的任何一种时，都应考虑戊巴比妥和异氟烷都会引起血压、心率、血管加压素、血浆肾素活性和通气驱动的变化。
