Russ G, Ruttkay-Nedecky G, Mucha V
Acta Virol. 1976 Feb;20(1):1-8.
Subunits released from influenza A/Singapore/1/57 (H2N2) virus by either Triton-X-100 (T-X-100); or sodium lauryl sarcosinate (SLS) or ether were separated by electrophoresis in agarose suspension into a rapidly migrating fraction (I) and a slowly migrating fraction (II). Fraction I obtained after T-X-100 treatment contained the viral ribonucleoprotein (RNP) in a form indistinguishable from the obtained after ether treatment. SLS treatment of the virus resulted in a rapidly migrating fraction containing only the protein part of the viral RNP. Fraction II obtained after T-X-100 or SLS treatment contained both haemagglutinin (HA) and neuraminidase (NA), mostly dissociated from each other, in contrast to fraction II obtained after ether treatment which contained mixed aggregates of HA and NA. The yields of electrophoretically isolated RNP and HA-NA were essentially the same irrespective of whether T-X-100 or ether was used for virus disruption. Treatment of virus by T-X-100 and subsequent removal of the latter resulted in a 10-20-fold increase of the HA activity. After sodium dodecyl sulphate (SDS) treatment of the virus, the NA activity was found in a heterogeneous fraction with surprisingly high migration rate towards the anode, indicating that NA remained active despite its extensive SDS binding.
通过Triton-X-100（T-X-100）、十二烷基肌氨酸钠（SLS）或乙醚从甲型流感病毒/新加坡/1/57（H2N2）中释放出的亚基，在琼脂糖悬浮液中通过电泳分离为快速迁移部分（I）和缓慢迁移部分（II）。T-X-100处理后获得的部分I含有病毒核糖核蛋白（RNP），其形式与乙醚处理后获得的无法区分。病毒经SLS处理后产生一个快速迁移部分，仅包含病毒RNP的蛋白质部分。T-X-100或SLS处理后获得的部分II含有血凝素（HA）和神经氨酸酶（NA），它们大多相互解离，这与乙醚处理后获得的部分II不同，后者含有HA和NA的混合聚集体。无论使用T-X-100还是乙醚进行病毒裂解，电泳分离的RNP和HA-NA的产量基本相同。用T-X-100处理病毒并随后去除后者，导致HA活性增加10至20倍。病毒经十二烷基硫酸钠（SDS）处理后，在一个异质部分中发现了NA活性，该部分向阳极的迁移率惊人地高，这表明尽管NA与大量SDS结合，但仍保持活性。
