Janz L, Hug M, Clayton C
Zentrum für Molekulare Biologie, Heidelberg, Germany.
Mol Biochem Parasitol. 1994 May;65(1):99-108. doi: 10.1016/0166-6851(94)90119-8.
The procyclic acidic repetitive protein (procyclin) and variant surface glycoprotein genes of Trypanosoma brucei are transcribed by a polymerase sharing many features with RNA polymerase I. Mutational analyses on the PARP and ribosomal RNA promoters have shown that sequences important for promoter activity are concentrated 20-60 bp upstream of the transcription initiation site. The results of gel mobility shift assays using synthetic oligonucleotides spanning of these regions indicated the presence in trypanosomal extracts of factors capable of binding each promoter in a highly specific fashion. There was no evidence that the PARP, VSG and rRNA promoter fragments bound the same factor.
布氏锥虫的前循环酸性重复蛋白（前循环素）和可变表面糖蛋白基因由一种与RNA聚合酶I具有许多共同特征的聚合酶转录。对PARP和核糖体RNA启动子的突变分析表明，对启动子活性重要的序列集中在转录起始位点上游20-60 bp处。使用跨越这些区域的合成寡核苷酸进行凝胶迁移率变动分析的结果表明，锥虫提取物中存在能够以高度特异性方式结合每个启动子的因子。没有证据表明PARP、VSG和rRNA启动子片段结合相同的因子。
