聚合酶链反应扩增DNA的单链化学测序：末端转移酶和碱基类似物次黄嘌呤的应用 - Suppr

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超能文献

One-lane chemical sequencing of PCR amplified DNA: the use of terminal transferase and of the base analogue inosine.

作者信息

Di Mauro E, Costanzo G, Negri R

机构信息

Centro di Studio per gli Acidi Nucleici (CNR), Rome, Italy.

出版信息

Nucleic Acids Res. 1994 Sep 11;22(18):3811-2. doi: 10.1093/nar/22.18.3811.

DOI:10.1093/nar/22.18.3811

PMID:7937097

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC308368/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b900/308368/74423e8a5154/nar00042-0153-a.jpg

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引用本文的文献

Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.使用T4 RNA连接酶对单链DNA/RNA的5'末端进行荧光、同位素或生物素标记。

Nucleic Acids Res. 1997 Sep 15;25(18):3747-8. doi: 10.1093/nar/25.18.3747.

本文引用的文献

Chemical method for DNA sequence determination from the 5'-extremity on PCR amplified fragments.从PCR扩增片段的5'末端进行DNA序列测定的化学方法。

Nucleic Acids Res. 1994 Jan 11;22(1):111-2. doi: 10.1093/nar/22.1.111.

One-lane chemical sequencing of 3'-fluorescent-labeled DNA.

Anal Biochem. 1993 Nov 1;214(2):566-70. doi: 10.1006/abio.1993.1539.

A single-reaction method for DNA sequence determination.一种用于DNA序列测定的单反应方法。

Anal Biochem. 1991 Sep 2;197(2):389-95. doi: 10.1016/0003-2697(91)90409-m.

An effective method for eliminating "artifact banding" when sequencing double-stranded DNA templates.

Biotechniques. 1990 Jul;9(1):46-8.

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