Ferraboli S, Negri R, Di Mauro E, Barlati S
Dipartimento di Scienze Biomediche e Biotechnologie, Università di Brescia, Italy.
Anal Biochem. 1993 Nov 1;214(2):566-70. doi: 10.1006/abio.1993.1539.
A method for DNA sequencing that combines limited chemical degradation of 3'-fluorescent-labeled DNA with densitometric analysis using an automatic sequencer is presented. The DNA sequence is determined in a single electrophoretic lane by monitoring the intensities of bands representing products of cleavage at the four bases obtained by solvolysis in hot aqueous piperidine (10%) followed by treatment with hot formamide. An application of the method for the detection of point mutations is reported.
