5-硝基吲哚作为一种通用的碱基类似物。
5-Nitroindole as an universal base analogue.
作者信息
Loakes D, Brown D M
机构信息
Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.
出版信息
Nucleic Acids Res. 1994 Oct 11;22(20):4039-43. doi: 10.1093/nar/22.20.4039.
Abstract
4-, 5- and 6-Nitroindole have been investigated and compared with 3-nitropyrrole as universal bases in oligodeoxynucleotides. Of these the 5-nitroindole derivative was found to be superior giving higher duplex stability, and behaving indiscriminately towards each of the four natural bases in duplex melting experiments. 3-Nitropyrrole, whilst not discriminating between the natural bases, was found to lead to considerable destabilisation of the duplexes, particularly when multiple substitutions were made, in contrast to the 5-nitroindole nucleoside.
摘要
已对4-、5-和6-硝基吲哚进行了研究，并将它们与3-硝基吡咯作为寡脱氧核苷酸中的通用碱基进行了比较。其中，发现5-硝基吲哚衍生物更具优势，能提供更高的双链稳定性，并且在双链解链实验中对四种天然碱基中的每一种表现无差别。3-硝基吡咯虽然对天然碱基没有区分，但与5-硝基吲哚核苷相比，发现它会导致双链体显著不稳定，尤其是在进行多次取代时。
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