Inoue H, Imura A, Ohtsuka E
Nucleic Acids Res. 1985 Oct 11;13(19):7119-28. doi: 10.1093/nar/13.19.7119.
Partially self-complementary dodecadeoxyribonucleotides containing a fluorescent nucleoside, 3-beta-D-2'-deoxyribofuranosyl-2, 7-dioxopyrido[2, 3-d]pyrimidine (pyridopyrimidine deoxynucleoside, dF) were synthesized by the phosphotriester solidphase method. A dodecanucleotide d(GGGAAFGTTCCC) pairing the analog and guanine at the centre of the chain showed a higher melting temperature than the corresponding G-C paired duplex. A similar comparison between A-T and A-F suggested that weaker hydrogen bonds exist when adenine and pyridopyrimidine residues are paired.
通过磷酸三酯固相法合成了含有荧光核苷3-β-D-2'-脱氧呋喃核糖基-2,7-二氧代吡啶并[2,3-d]嘧啶（吡啶并嘧啶脱氧核苷，dF）的部分自互补十二脱氧核糖核苷酸。在链中心将该类似物与鸟嘌呤配对的十二核苷酸d(GGGAAFGTTCCC)的解链温度高于相应的G-C配对双链体。A-T和A-F之间的类似比较表明，当腺嘌呤和吡啶并嘧啶残基配对时存在较弱的氢键。
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