一种作为模糊核苷的无环5-硝基吲唑核苷类似物。
An acyclic 5-nitroindazole nucleoside analogue as ambiguous nucleoside.
作者信息
Van Aerschot A, Rozenski J, Loakes D, Pillet N, Schepers G, Herdewijn P
机构信息
Laboratory of Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Belgium.
出版信息
Nucleic Acids Res. 1995 Nov 11;23(21):4363-70. doi: 10.1093/nar/23.21.4363.
Abstract
Acyclic nucleoside analogues with carboxamido- or nitro-substituted heterocyclic bases have been evaluated for their possible use as universal bases in oligodeoxynucleotides. The acyclic moiety endows the constructs with enough flexibility to allow good base stacking. The 5-nitroindazole analogue afforded the most stable duplexes among the acyclic derivatives with the least spread in Tm versus the four natural bases. In spite of the acyclic moiety, stabilities are comparable with those of duplexes incorporating the recently described 5-nitroindole nucleoside analogue, but considerably exceed those for the 3-nitropyrrole analogue.
摘要
已对具有羧酰胺基或硝基取代杂环碱基的无环核苷类似物作为寡脱氧核苷酸通用碱基的潜在用途进行了评估。无环部分赋予这些构建体足够的灵活性，以实现良好的碱基堆积。在无环衍生物中，5-硝基吲唑类似物形成的双链体最稳定，其与四种天然碱基相比，熔解温度（Tm）的差异最小。尽管存在无环部分，其稳定性与掺入最近描述的5-硝基吲哚核苷类似物的双链体相当，但大大超过了3-硝基吡咯类似物的稳定性。
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