来自氨基戊酸梭菌的绿色黄素酶5-羟基戊酰辅酶A脱水酶/脱氢酶的结晶及初步X射线衍射研究

Crystallization and preliminary X-ray diffraction study of the green flavoenzyme 5-hydroxyvaleryl-CoA dehydratase/dehydrogenase from Clostridium aminovalericum.

作者信息

Eikmanns U, Buta C, Buckel W, Pai E F

机构信息

Department of Biochemistry, University of Toronto, Ontario, Canada.

出版信息

Proteins. 1994 Jul;19(3):269-71. doi: 10.1002/prot.340190310.

DOI:10.1002/prot.340190310

PMID:7937739

Abstract

The bifunctional flavoenzyme 5-hydroxyvaleryl-CoA dehydratase/dehydrogenase has been crystallized from solutions containing ammonium sulfate (form I) or polyethylene glycol (form II) as precipitant. In both cases, the crystals grew in the monoclinic space group C2. The unit cell dimensions for form I crystals were determined as a = 162.8 A, b = 71.8 A, c = 83.5 A, beta = 109.1 degrees; corresponding values for form II crystals were a = 161.2 A, b = 71.6 A, c = 82.2 A, beta = 109.3 degrees. In both cases most probably there are two monomers per asymmetric unit. The crystals diffract to about 2 A resolution and are rather stable in the X-ray beam.

摘要

双功能黄素酶5-羟基戊酰辅酶A脱水酶/脱氢酶已从含有硫酸铵（晶型I）或聚乙二醇（晶型II）作为沉淀剂的溶液中结晶出来。在这两种情况下，晶体均在单斜空间群C2中生长。晶型I晶体的晶胞尺寸确定为a = 162.8 Å，b = 71.8 Å，c = 83.5 Å，β = 109.1°；晶型II晶体的相应值为a = 161.2 Å，b = 71.6 Å，c = 82.2 Å，β = 109.3°。在这两种情况下，每个不对称单元很可能有两个单体。这些晶体的衍射分辨率约为2 Å，并且在X射线束中相当稳定。