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在携带抗病基因Cf-9的番茄幼苗中，真菌无毒基因Avr9表达时的发育调控细胞死亡。

Developmentally regulated cell death on expression of the fungal avirulence gene Avr9 in tomato seedlings carrying the disease-resistance gene Cf-9.

作者信息

Hammond-Kosack K E, Harrison K, Jones J D

机构信息

Sainsbury Laboratory, John Innes Centre, Norwich, Norfolk, United Kingdom.

出版信息

Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10445-9. doi: 10.1073/pnas.91.22.10445.

DOI:10.1073/pnas.91.22.10445
PMID:7937972
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC45037/
Abstract

Plant defense responses are induced when the products of disease-resistance genes and pathogen avirulence genes interact. We report here the effects of expressing the Cladosporium fulvum avirulence Avr9 gene product in a tomato line containing the Cf-9 disease-resistance gene. A synthetic Avr9 gene was constructed to produce constitutive high-level expression of AVR9 peptide in the plant apoplast. Avr9 expression in Cf-9-containing tomato lines is lethal, but cell death is developmentally regulated, in that necrosis is not visible until 10 days after planting seed. Plant lines lacking Cf-9 and expressing Avr9 remain healthy. The synthetic Avr9 gene exhibited the same specificity of action as the authentic C. fulvum Avr9 gene. Our results have significant implications for strategies using the gene combination Avr9/Cf-9 to engineer plants with enhanced disease resistance.

摘要

当抗病基因产物与病原菌无毒基因产物相互作用时，植物的防御反应就会被诱导。我们在此报告在含有Cf - 9抗病基因的番茄品系中表达番茄叶霉病菌无毒基因Avr9的产物的效果。构建了一个合成Avr9基因，以在植物质外体中组成型高水平表达AVR9肽。在含有Cf - 9的番茄品系中Avr9的表达是致死性的，但细胞死亡受发育调控，因为直到播种后10天坏死才可见。缺乏Cf - 9并表达Avr9的植物品系保持健康。合成的Avr9基因表现出与正宗的番茄叶霉病菌Avr9基因相同的作用特异性。我们 的结果对于利用Avr9/Cf - 9基因组合培育抗病性增强的植物的策略具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6720/45037/d3d428c9bf44/pnas01144-0215-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6720/45037/ef873d8f9908/pnas01144-0214-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6720/45037/ac5d18405cf0/pnas01144-0214-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6720/45037/73f5dfa4c25a/pnas01144-0215-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6720/45037/d3d428c9bf44/pnas01144-0215-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6720/45037/ef873d8f9908/pnas01144-0214-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6720/45037/ac5d18405cf0/pnas01144-0214-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6720/45037/73f5dfa4c25a/pnas01144-0215-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6720/45037/d3d428c9bf44/pnas01144-0215-b.jpg

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