Tucker J D, Senft J R
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, California 94551.
Radiat Res. 1994 Oct;140(1):31-6.
The locations of observed breaks in three pairs of painted chromosomes from radiation-exposed and unexposed human peripheral blood lymphocytes are described. No difference in the observed breakpoint locations was seen from people exposed at Chernobyl, from healthy controls or from blood exposed to 2 Gy 137Cs in vitro. However, the distribution of observed breaks within the painted chromosomes was not random. Fewer breaks and rearrangements were observed near the ends of the chromosome arms. Three explanations for these findings were considered: cell selection, non-random efficiency of detection and non-random breakage or repair. Cell selection does not appear to be plausible because the distribution of observed breaks induced in vitro is not different from those induced in vivo. Non-random efficiency of detection is not supported by the data. Non-random breakage or repair appears to be the most likely explanation, although the mechanism(s) by which this occurs is unknown.
本文描述了在受辐射和未受辐射的人类外周血淋巴细胞中三对涂染染色体上观察到的断裂位置。切尔诺贝利事故受害者、健康对照者以及体外接受2 Gy 137Cs照射的血液样本，在观察到的断裂点位置上没有差异。然而，涂染染色体上观察到的断裂分布并非随机。在染色体臂末端附近观察到的断裂和重排较少。对于这些发现，考虑了三种解释：细胞选择、非随机检测效率以及非随机断裂或修复。细胞选择似乎不太合理，因为体外诱导的观察到的断裂分布与体内诱导的并无不同。数据不支持非随机检测效率这一解释。非随机断裂或修复似乎是最有可能的解释，尽管其发生机制尚不清楚。
