Salín-Pascual R J, Jiménez-Anguiano A, Durán-Vazquez A, Nancy H M, Drucker-Colín R
Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autonoma de México, México City.
Sleep. 1994 Apr;17(3):231-5. doi: 10.1093/sleep/17.3.231.
Rapid eye movement (REM) sleep deprivation and auditory stimulation (ADS), separately, increase REM sleep in rats, cats and humans. The main goal of the present study was to test whether administration of ADS during REM sleep rebound has a synergistic effect on REM sleep elicitation. Male Wistar rats were implanted with standard sleep recording electrodes. Following the recovery period, animals were randomly assigned to the following conditions: undeprived (i.e. control) and 24, 48, 96 and 120 hours of REM sleep deprivation by the platform method. Undeprived and REM sleep-deprived animals were divided into two groups, with and without ADS. ADS was a "beep" of 80 dB and 2,000 Hz, lasting 20 msec every 10 seconds. This stimulus was applied for the first 4 hours of sleep recordings after deprivation. After that, animals were recorded for another 4 hours. In the undeprived situation, the group that received ADS increased REM sleep approximately 70% above the group that did not receive ADS, as has been reported previously (REM sleep without ADS: 38.1 +/- 13.84 vs. with ADS: 64.6 +/- 11.8, p < 0.005). No synergistic effect was observed between REM sleep deprivation and ADS for any REM sleep-deprivation schedule. This result may be explained as an increase in the excitability pattern of pontine neurons and/or changes in the cholinergic system due to REM sleep deprivation that could not be further increased by ADS.
快速眼动（REM）睡眠剥夺和听觉刺激（ADS）分别会增加大鼠、猫和人类的快速眼动睡眠。本研究的主要目的是测试在快速眼动睡眠反弹期间给予听觉刺激是否对快速眼动睡眠的诱发有协同作用。雄性Wistar大鼠植入标准睡眠记录电极。在恢复期后，动物被随机分配到以下条件：未剥夺组（即对照组）以及通过平台法剥夺24、48、96和120小时快速眼动睡眠的组。未剥夺组和快速眼动睡眠剥夺组的动物又被分为两组，一组有听觉刺激，一组没有。听觉刺激是80分贝、2000赫兹的“哔哔”声，每10秒持续20毫秒。该刺激在剥夺后睡眠记录的前4小时施加。之后，对动物再记录4小时。在未剥夺的情况下，接受听觉刺激的组的快速眼动睡眠比未接受听觉刺激的组增加了约70%，如先前报道的那样（无听觉刺激的快速眼动睡眠：38.1±13.84 vs. 有听觉刺激的：64.6±11.8，p<0.005）。对于任何快速眼动睡眠剥夺时间表，在快速眼动睡眠剥夺和听觉刺激之间均未观察到协同作用。这一结果可能解释为，由于快速眼动睡眠剥夺导致脑桥神经元兴奋性模式增加和/或胆碱能系统发生变化，而听觉刺激无法进一步增强这种变化。
