2,4,6-三硝基苯磺酸诱导的大鼠慢性结肠炎愈合过程，特别提及纤连蛋白的作用

The healing process of chronic colitis in rats, induced by 2,4,6-trinitrobenzene sulfonic acid, with special reference to the role of fibronectin.

作者信息

Hirata K, Nagata N, Hiranuma K, Hirano H, Osaka T, Itoh H, Ohsato K

机构信息

Dept of Surgery 1, University of Occupational and Environmental Health, Kitakyushu, Japan.

出版信息

Scand J Gastroenterol. 1994 Jul;29(7):624-9. doi: 10.3109/00365529409092483.

DOI:10.3109/00365529409092483

PMID:7939399

Abstract

BACKGROUND

The healing process of rat chronic colitis was investigated to evaluate the role of fibronectin produced by fibroblasts in the healing tissue.

METHODS

Chronic colitis was produced by intrarectal instillation of 2,4,6-trinitrobenzene sulfonic acid. The healing process was observed by light and electron microscopy and immunocytochemistry. Time course of fibronectin mRNA expression was measured by Northern blot hybridization.

RESULTS

By light and electron microscopy, an abundance of young fibroblasts had gathered at the healing tissue and were involved in incorporation into the endothelium of the preexisting vessels, as vasoformative cells, and in transformation to new muscle cells in muscular regeneration. Such processes became pronounced from day 3 and persisted for 6 weeks. Immunocytochemical data showed that fibronectin, produced in the rough endoplasmic reticulum of the fibroblasts and released to the extracellular surface, played inductive roles in the movement and aggregation of such cells and in their contacts with neighboring endothelial cells of the preexisting capillaries. Northern hybridization showed that mRNA coding for fibronectin rapidly reached maximum on day 3 and gradually decreased almost to the control level at 6 weeks.

CONCLUSIONS

These observations elucidate the role of young fibroblasts, as multipotent cells, and of fibronectin in acceleration of the healing process in this model of chronic colitis.

摘要

背景

研究大鼠慢性结肠炎的愈合过程，以评估成纤维细胞产生的纤连蛋白在愈合组织中的作用。

方法

通过直肠内注入2,4,6-三硝基苯磺酸诱导产生慢性结肠炎。通过光镜、电镜和免疫细胞化学观察愈合过程。采用Northern印迹杂交法检测纤连蛋白mRNA表达的时间进程。

结果

光镜和电镜观察显示，大量年轻成纤维细胞聚集在愈合组织中，作为血管形成细胞参与并入原有血管的内皮，并在肌肉再生过程中转化为新的肌细胞。这些过程从第3天开始明显，并持续6周。免疫细胞化学数据显示，在成纤维细胞糙面内质网中产生并释放到细胞外表面的纤连蛋白，在此类细胞的移动和聚集以及它们与原有毛细血管相邻内皮细胞的接触中发挥诱导作用。Northern杂交显示，编码纤连蛋白的mRNA在第3天迅速达到最大值，并在6周时逐渐下降至几乎对照水平。