Theodorsen L
Central Laboratory, Norwegian Radium Hospital, Oslo, Norway.
Clin Lab Haematol. 1995 Sep;17(3):225-9.
Accurate determination of monocytes can be a problem with automated haematological analysers as well as with visual microscopy. We have compared monocyte counts from our two routine automated instruments, Technicon H1 and Sysmex NE-1500. In the NE-1500 the monocyte counts appear to increase with increasing concentrations of neutrophils, compared with the H1 results. The accuracy of the monocyte counts was assessed using Dynabeads M-450 CD14. These uniform magnetizable polystyrene beads coated with monoclonal antibody specific for CD14 membrane antigen isolated monocytes from the blood samples. The influence of this depletion on the monocyte counts and histograms from the two instruments and by visual microscopy were compared. On average 90% of the monocytes counted by visual microscopy were removed from six blood samples. In the same samples the beads removed an average of 75% of cells identified as monocytes by the H*1 but only 49% of those identified as monocytes by the NE-1500. These Dynabeads can be a useful tool in evaluating monocyte identification in different haematological situations and for manufacturers developing technology for automated leucocyte differential counting.
对于自动血液分析仪以及目视显微镜检查而言，准确测定单核细胞都可能存在问题。我们比较了我们两台常规自动仪器Technicon H1和Sysmex NE - 1500的单核细胞计数结果。与H1的结果相比，在NE - 1500中，单核细胞计数似乎随着中性粒细胞浓度的增加而增加。使用Dynabeads M - 450 CD14评估单核细胞计数的准确性。这些均匀的可磁化聚苯乙烯珠包被有针对CD14膜抗原的单克隆抗体，用于从血样中分离单核细胞。比较了这种去除方法对两台仪器的单核细胞计数和直方图以及目视显微镜检查结果的影响。通过目视显微镜检查，平均从六个血样中去除了90%计数的单核细胞。在相同的样本中，这些磁珠从H*1鉴定为单核细胞的细胞中平均去除了75%，但从NE - 1500鉴定为单核细胞的细胞中仅去除了49%。这些Dynabeads可作为一种有用的工具，用于评估不同血液学情况下单核细胞识别情况，以及供制造商开发自动白细胞分类计数技术。
