Xiao W, Penugonde V, Rank G H
Department of Microbiology, University of Saskatchewan, Saskatoon, Canada.
Yeast. 1994 May;10(5):687-91. doi: 10.1002/yea.320100513.
The MAG1 gene encodes a 3-methyladenine DNA glycoslyase, which is involved in DNA alkylation repair in Saccharomyces cerevisiae. The mag1 mutant is deficient in 3-methyladenine DNA glycosylase activity and shows enhanced sensitivity to several monofunctional alkylating agents. MAG1 is allelic to MMS5. This gene has been previously located on chromosome V by chromosomal hybridization. We present physical and genetic mapping data here showing that the MAG1 gene is located on chromosome V-R, proximal to and about 10 kilobase pairs away from the SPT15 gene coding for the yeast TATA-binding protein TFIID.
MAG1基因编码一种3-甲基腺嘌呤DNA糖基化酶，它参与酿酒酵母中的DNA烷基化修复。mag1突变体缺乏3-甲基腺嘌呤DNA糖基化酶活性，并且对几种单功能烷基化剂表现出增强的敏感性。MAG1与MMS5等位。该基因先前已通过染色体杂交定位在第五条染色体上。我们在此呈现物理和遗传图谱数据，表明MAG1基因位于第五条染色体的右臂上，靠近编码酵母TATA结合蛋白TFIID的SPT15基因，且距离该基因约10千碱基对。
