Paoluzi S, Minenkova O, Castagnoli L
Department of Biology, University of Rome, Tor Vergata, Italy.
Yeast. 1997 Jan;13(1):85-91. doi: 10.1002/(SICI)1097-0061(199701)13:1<85::AID-YEA53>3.0.CO;2-E.
We report the nucleotide sequence of a DNA fragment of 12,325 base pairs from the left arm of the Saccharomyces cerevisiae chromosome VII. Inspection of the coding capacity revealed 11 open reading frames (ORFs) longer than 100 amino acids. Five ORFs are significantly homologous to known proteins. The region encoding ORF G2985 corresponds (100%) to the gene encoding the yeast TATA binding protein-associated factor TAFII60. The G3075 ORF is 47.8% identical to the hypothetical yeast protein YB88. G3089 shows 36.7% identity to the eel calmodulin. G3085 shows 94.9% identity with the published sequence of the quadruplex DNA binding protein G4p1. G3090 reveals 46.7% identity with the probable glucose transport protein yBR1625.
我们报道了来自酿酒酵母七号染色体左臂的一段12325个碱基对的DNA片段的核苷酸序列。对编码能力的检查发现有11个开放阅读框(ORF)长度超过100个氨基酸。其中5个ORF与已知蛋白质具有显著的同源性。编码ORF G2985的区域(100%)对应于编码酵母TATA结合蛋白相关因子TAFII60的基因。ORF G3075与假定的酵母蛋白YB88有47.8%的同一性。G3089与鳗鱼钙调蛋白有36.7%的同一性。G3085与已发表的四链体DNA结合蛋白G4p1的序列有94.9%的同一性。G3090与可能的葡萄糖转运蛋白yBR1625有46.7%的同一性。