水痘带状疱疹病毒抗原-抗体复合物对人多形核白细胞产生过氧化氢的影响。

Effect of varicella zoster virus antigen-antibody complexes on hydrogen peroxide generation by human polymorphonuclear leukocytes.

作者信息

Fujiwara T, Ihara T, Yamawaki K, Ito M, Kamiya H, Sakurai M

机构信息

Department of Pediatrics, Mie University, Japan.

出版信息

Acta Paediatr Jpn. 1994 Aug;36(4):341-6. doi: 10.1111/j.1442-200x.1994.tb03198.x.

DOI:10.1111/j.1442-200x.1994.tb03198.x

PMID:7941996

Abstract

Hydrogen peroxide (H2O2) generation by human polymorphonuclear leukocytes (PMN) incubated with varicella zoster virus (VZV) antigen was studied by cytofluorography. Hydrogen peroxide generation was detected in the presence of VZV-seropositive sera. When seropositive sera were heat-inactivated, H2O2 generation was reduced. When PMN were pre-incubated with Leu-11b, a monoclonal antibody to the Fc receptor on PMN, H2O2 generation was also reduced. These results suggest that VZV antigen-antibody-complement complexes induce H2O2 generation by PMN after these complexes attach to Fc receptors on PMN.

摘要

采用细胞荧光成像法研究了人多形核白细胞（PMN）与水痘带状疱疹病毒（VZV）抗原一起孵育时过氧化氢（H2O2）的生成情况。在VZV血清反应阳性的血清存在下可检测到过氧化氢的生成。当血清反应阳性的血清经热灭活后，H2O2的生成减少。当PMN与Leu-11b（一种针对PMN上Fc受体的单克隆抗体）预孵育时，H2O2的生成也减少。这些结果表明，VZV抗原-抗体-补体复合物在附着于PMN上的Fc受体后可诱导PMN生成H2O2。