Fujiwara T, Ihara T, Yamawaki K, Kamiya H, Sakurai M
Department of Pediatrics, Mie National Hospital, ,357 Ohsato-Kubota, Tsu, Mie, 514-01, Japan.
Clin Diagn Virol. 1994 Aug;2(4-5):221-9. doi: 10.1016/0928-0197(94)90047-7.
In varicella-zoster virus (VZV) infections, the initial response in cutaneous lesions is predominantly polymorphonuclear leukocytes (PMN). However, interaction of VZV and PMN has not yet been fully defined.
To explore the potential role of PMN in VZV infections, we investigated the ability of PMN to generate hydrogen peroxide (H(2)O(2)) after stimulation with VZV antigen.
H(2)O(2), one of the reactive oxygen species, was measured by cytofluorography after PMN were incubated with varicella-zoster virus (VZV) antigen in the presence of seropositive or seronegative serum.
H(2)O(2) was detectable in unseparated PMN obtained from seropositive children and adults, while unseparated PMN of seronegative donors were not capable of generating H(2)O(2). Separated PMN obtained from either seropositive or seronegative donors were capable of generating H(2)O(2) in the presence of seropositive serum. However, H(2)O(2) was not detectable in separated PMN of seropositive donors in the presence of seronegative serum. The of H(2)O(2) generation did not correlate with concentrations of serum antibodies to VZV.
VZV antigen-antibody complexes might induce H(2)O(2) generation by PMN.
在水痘 - 带状疱疹病毒（VZV）感染中，皮肤损伤处的初始反应主要是多形核白细胞（PMN）。然而，VZV与PMN之间的相互作用尚未完全明确。
为了探究PMN在VZV感染中的潜在作用，我们研究了PMN在VZV抗原刺激后产生过氧化氢（H₂O₂）的能力。
在血清阳性或血清阴性的血清存在下，将PMN与水痘 - 带状疱疹病毒（VZV）抗原孵育后，通过细胞荧光术测量活性氧之一的H₂O₂。
在从血清阳性儿童和成人获得未分离的PMN中可检测到H₂O₂，而血清阴性供体的未分离PMN不能产生H₂O₂。在血清阳性血清存在下，从血清阳性或血清阴性供体获得的分离PMN能够产生H₂O₂。然而，在血清阴性血清存在下，血清阳性供体的分离PMN中未检测到H₂O₂。H₂O₂的产生与抗VZV血清抗体浓度无关。
VZV抗原 - 抗体复合物可能诱导PMN产生H₂O₂。
