Pettersson H, Kiessling K H
J Assoc Off Anal Chem. 1984 May-Jun;67(3):503-6.
A simplified procedure is described for the cleanup and determination of the 5 main plant estrogens in animal feed: coumestrol, genistein, biochanin A, daidzein, and formononetin. The estrogens are extracted with ethanol and purified on a Sep-Pak C18 cartridge. All 5 plant estrogens are separated in an isocratic liquid chromatographic system and quantitated by UV and fluorescence responses. The method is sensitive to about 2.5 ppm coumestrol and 10 ppm of the isoflavones.
本文描述了一种用于动物饲料中5种主要植物雌激素（香豆雌酚、染料木黄酮、鹰嘴豆芽素A、大豆苷元、芒柄花黄素）净化与测定的简化方法。这些雌激素用乙醇提取，并在Sep-Pak C18柱上进行纯化。所有5种植物雌激素在等度液相色谱系统中分离，并通过紫外和荧光响应进行定量。该方法对香豆雌酚约2.5 ppm以及异黄酮10 ppm敏感。
