Deutsch J C, Kolli V R, Santhosh-Kumar C R, Kolhouse J F
Division of Gastroenterology, University of Colorado Health Sciences Center, Denver 80262.
Am J Clin Pathol. 1994 Nov;102(5):595-9. doi: 10.1093/ajcp/102.5.595.
A gas chromatography/mass spectrometric (GC/MS) isotope dilution assay for xylose was developed using tertbutyldimethylsilyl-derivatized xylose and [13C]1xylose, and applied to human serum samples. A calibration curve in serum using this assay showed < 3% variation (< 10 mg/L) for any given point. The correlation coefficient for xylose measurements made on 27 sera between a colorimetric method performed by a national commercial reference laboratory and the GC/MS method developed here was .952. However, xylose determinations of 10 of 27 samples differed by > 10% (up to 150 mg/L) when colorimetric values were compared to GC/MS. Two of these samples had borderline-low xylose values by GC/MS, but were well within the normal range by colorimetric analysis. gas chromatography/mass spectrometric isotope dilution assay appears to be an accurate method to measure xylose in serum. These data also suggest that further prospective studies comparing GC/MS to colorimetric methods are indicated for subjects undergoing oral xylose testing.
使用叔丁基二甲基甲硅烷基衍生化木糖和[¹³C]₁木糖开发了一种用于木糖的气相色谱/质谱（GC/MS）同位素稀释测定法，并将其应用于人体血清样本。使用该测定法在血清中绘制的校准曲线显示，任何给定浓度下的变异<3%（<10mg/L）。由一家国家级商业参考实验室采用的比色法与这里开发的GC/MS方法对27份血清中的木糖进行测定，其相关系数为0.952。然而，将比色法测定值与GC/MS法测定值相比较时，27个样本中有10个样本的木糖测定值差异>10%（高达150mg/L）。其中两个样本通过GC/MS法测定的木糖值处于临界低值，但通过比色分析则完全在正常范围内。气相色谱/质谱同位素稀释测定法似乎是一种准确测定血清中木糖的方法。这些数据还表明，对于接受口服木糖测试的受试者，需要进行进一步的前瞻性研究，将GC/MS法与比色法进行比较。
