Inoue M, Fujita M, Enomoto T, Morimoto H, Monden T, Shimano T, Tanizawa O
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Am J Clin Pathol. 1994 Nov;102(5):665-70. doi: 10.1093/ajcp/102.5.665.
Immunohistochemical staining for the p53 protein was performed in microwave-fixed, paraffin-embedded sections of normal, premalignant and malignant tissues of the female genital tract using a monoclonal antibody, PAb 1801. No staining was detected in normal and premalignant tissues, whereas nuclear staining of cancer cells was observed in 12 (22%) of 55 cervical squamous cell carcinomas, 4 (25%) of 16 cervical adenocarcinomas, 37 (42%) of 88 endometrial carcinomas, 23 (38%) of 60 ovarian adenocarcinomas, and 6 (100%) of 6 squamous cell carcinomas arising in dermoid cysts. Of interest, 1 of 7 endometrial cancers with concomitant atypical hyperplasia showed weak nuclear staining in a few atypical hyperplastic glands in addition to the cancerous lesions. Although staining was associated with cancers having a high histologic grade and serous papillary adenocarcinomas of the endometrium, it did not correlate with invasion, metastasis, or clinical stage. Comparison of the staining patterns with molecular analysis of mutations in the p53 gene showed the expected correlation of nuclear staining with missense mutations but not with nonsense mutations, which consistuted one third of all mutations found in this series. In addition, cytoplasmic staining did not predict mutation.
采用单克隆抗体PAb 1801，对女性生殖道正常、癌前和恶性组织的微波固定石蜡包埋切片进行p53蛋白的免疫组织化学染色。在正常和癌前组织中未检测到染色，而在55例宫颈鳞状细胞癌中有12例（22%）、16例宫颈腺癌中有4例（25%）、88例子宫内膜癌中有37例（42%）、60例卵巢腺癌中有23例（38%）以及6例皮样囊肿中发生的鳞状细胞癌中有6例（100%）观察到癌细胞的核染色。有趣的是，7例伴有非典型增生的子宫内膜癌中有1例，除癌性病变外，在少数非典型增生腺体中显示出弱核染色。虽然染色与高组织学分级的癌症以及子宫内膜浆液性乳头状腺癌有关，但它与浸润、转移或临床分期无关。将染色模式与p53基因突变的分子分析进行比较，结果显示核染色与错义突变存在预期的相关性，但与无义突变无关，无义突变占该系列中所有突变的三分之一。此外，细胞质染色不能预测突变。
