Kremlev S G, Umstead T M, Phelps D S
Department of Pediatrics, Pennsylvania State University, College of Medicine, Hershey 17033.
Am J Physiol. 1994 Oct;267(4 Pt 1):L357-64. doi: 10.1152/ajplung.1994.267.4.L357.
We studied the effects of dipalmitoyl L-alpha-phosphatidylcholine (DPPC), Survanta, surfactant protein A (SP-A), and mixtures of these substances on mitogen-induced lymphocyte proliferation using concanavalin A as a mitogen. A concentration-dependent suppression of proliferation was observed with 50-250 micrograms/ml of DPPC or Survanta. However, when SP-A was added to cultures, proliferation was stimulated. The inhibitory effects of DPPC and Survanta were altered in mixtures that contained SP-A. When added to 50 micrograms/ml of Survanta, SP-A reversed the inhibitory influence of Survanta and caused increased proliferation. These findings suggest that surfactant phospholipids cause a suppression of mitogen-induced lymphocyte proliferation, which is reversed somewhat by addition of SP-A. We hypothesize that immune cell function in the lung varies with changes in the relative amounts of surfactant components. Changes in surfactant composition may occur during pulmonary inflammation or infection or with surfactant replacement therapy and may influence immune and inflammatory processes in the lung.
我们使用伴刀豆球蛋白A作为促有丝分裂原，研究了二棕榈酰-L-α-磷脂酰胆碱（DPPC）、固尔苏、表面活性蛋白A（SP-A）以及这些物质的混合物对促有丝分裂原诱导的淋巴细胞增殖的影响。观察到50-250微克/毫升的DPPC或固尔苏对增殖有浓度依赖性抑制作用。然而，当将SP-A添加到培养物中时，增殖受到刺激。在含有SP-A的混合物中，DPPC和固尔苏的抑制作用发生了改变。当添加到50微克/毫升的固尔苏中时，SP-A逆转了固尔苏的抑制作用并导致增殖增加。这些发现表明，表面活性磷脂会抑制促有丝分裂原诱导的淋巴细胞增殖，而添加SP-A会在一定程度上逆转这种抑制作用。我们推测，肺中免疫细胞的功能会随着表面活性物质成分相对含量的变化而改变。表面活性物质组成的变化可能发生在肺部炎症或感染期间，或在表面活性物质替代治疗时，并且可能会影响肺部的免疫和炎症过程。
