Woerndle S, Bartmann P
Department of Pediatrics, University of Ulm, Fed. of Germany.
J Perinat Med. 1994;22(2):119-28. doi: 10.1515/jpme.1994.22.2.119.
Respiratory distress can be successfully treated by endotracheal surfactant replacement therapy. The effects of two natural surfactant preparations, Alveofact and Survanta, and one synthetic, Exosurf, on lymphocyte functions were investigated. Peripheral blood lymphocytes from cord blood or buffy coat from adult healthy donors were stimulated with mitogens (PHA-P, Con A), allogenic cells (MLC) or antigen (SEB), and the proliferative response was measured by the uptake of tritiated thymidine. Immunoglobulin synthesis was stimulated by PWM and determined by an ELISA. Stimulation of lymphocytes in the presence of variable surfactant concentrations resulted in a concentration dependent suppression of the investigated lymphocyte function, with different results for the three products. Complex immunological function such as proliferation in response to allogenic cells were more sensitive to surfactant induced suppression than mitogen induced proliferation. Thus, it can not be excluded that surfactant replacement therapy may influence the local immune response of the lung.
气管内表面活性剂替代疗法可成功治疗呼吸窘迫。研究了两种天然表面活性剂制剂（Alveofact和Survanta）以及一种合成表面活性剂（Exosurf）对淋巴细胞功能的影响。用丝裂原（PHA - P、Con A）、同种异体细胞（MLC）或抗原（SEB）刺激来自脐带血或成年健康供体血沉棕黄层的外周血淋巴细胞，并通过氚标记胸腺嘧啶核苷的摄取来测量增殖反应。用PWM刺激免疫球蛋白合成，并通过ELISA进行测定。在不同表面活性剂浓度存在的情况下刺激淋巴细胞会导致所研究的淋巴细胞功能受到浓度依赖性抑制，三种产品的结果不同。诸如对同种异体细胞反应的增殖等复杂免疫功能比丝裂原诱导的增殖对表面活性剂诱导的抑制更敏感。因此，不能排除表面活性剂替代疗法可能影响肺部局部免疫反应。
