O'Keeffe M J, O'Keeffe M, Glennon J D, Lightfield A R, Maxwell R J
Teagasc, National Food Centre, Castleknock, Dublin, Ireland.
Analyst. 1998 Dec;123(12):2711-4. doi: 10.1039/a805042b.
The development of a supercritical fluid extraction procedure for the extraction of clenbuterol from bovine liver tissue is described. The procedure involves a combination of supercritical fluid extraction with enzyme immunoassay for the determination of clenbuterol residues at the low ppb level. Method validation incorporating inter- and intra-assays was carried out on fortified liver tissue and showed good recoveries and low variations (RSD < 15%) for levels of clenbuterol of 0.5, 2 and 5 ppb. The developed procedure was shown to be successful for the determination of clenbuterol in incurred liver tissue. The effects of organic modifiers and of inherent sample moisture on analyte extractability are also presented.
本文描述了一种从牛肝组织中提取克伦特罗的超临界流体萃取方法。该方法将超临界流体萃取与酶免疫分析法相结合，用于测定低至十亿分之一水平的克伦特罗残留量。在强化肝组织上进行了包含批间和批内分析的方法验证，结果表明，对于0.5、2和5 ppb的克伦特罗水平，回收率良好且变异系数低（相对标准偏差<15%）。所开发的方法已成功用于测定实际受污染肝组织中的克伦特罗。文中还介绍了有机改性剂和样品固有水分对分析物可萃取性的影响。
