使用免疫印迹法提高对牛Ⅱ类DRβ链多态性的鉴别

Improved discrimination of bovine class II DR beta-chains polymorphisms using immunoblotting.

作者信息

Knowles G, Dutia B M, Glass E J, MacCarthy-Morrogh L, Spooner R L, Hopkins J

机构信息

Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, University of Edinburgh, UK.

出版信息

Anim Genet. 1994 Jun;25(3):129-31. doi: 10.1111/j.1365-2052.1994.tb00100.x.

DOI:10.1111/j.1365-2052.1994.tb00100.x

PMID:7943945

Abstract

An immunoblotting technique is reported that reveals electrophoretic variants in the beta-chains of class II antigens of the bovine major histocompatibility complex. One monoclonal antibody, mAb VPM57, reacted on immunoblots with an epitope present in approximately half of the haplotypes investigated. This reagent is especially useful in discriminating electrophoretic variants that have similar isoelectric points.

摘要

据报道，一种免疫印迹技术可揭示牛主要组织相容性复合体II类抗原β链中的电泳变体。一种单克隆抗体mAb VPM57在免疫印迹上与所研究的大约一半单倍型中存在的一个表位发生反应。该试剂在区分具有相似等电点的电泳变体方面特别有用。