Bailey E, Lear T L
Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington 40546-0099.
Anim Genet. 1994 Jun;25 Suppl 1:105-8. doi: 10.1111/j.1365-2052.1994.tb00414.x.
We compared pools of DNA from 10 Thoroughbred horses and 10 Arabian horses for the presence of randomly amplified polymorphic DNA (RAPD) markers which might be useful in distinguishing between the breeds. Using 212 decamer oligonucleotides and our polymerase chain reaction (PCR) conditions, 173 of the primers produced scoreable bands. The number of bands ranged from 0 to 9 with an average of 3.6. In family studies using 11 arbitrarily selected primers, five of the 11 primers produced polymorphic bands which exhibited Mendelian inheritance as dominant markers. When comparing the pooled DNA from Thoroughbred and Arabian horses we found 10 primers which identified markers present in the pooled DNA from one breed but absent in the pool from the other breed. Testing individual horses revealed that only two markers were wholly absent for one group while being present among members of the other. Primer UBC-85 (5'-GTGCTCGTGC-3') detected a pair of markers absent in Thoroughbred horses but present among 11 of 31 Arabian horses. These markers were 1500 and 1700 base pairs (bp) long and designated UBC-85C and UBC-85D, respectively. Primer UBC-126 (5'-CTTTCGTGCT-3') detected a 1000 bp marker (designated UBC-126C) absent in 20 of 20 Thoroughbred horses but present in 31 of 31 Arabian horses.(ABSTRACT TRUNCATED AT 250 WORDS)
我们比较了10匹纯种马和10匹阿拉伯马的DNA池，以寻找可能有助于区分这两个品种的随机扩增多态性DNA（RAPD）标记。使用212种十聚体寡核苷酸和我们的聚合酶链反应（PCR）条件，173种引物产生了可计分的条带。条带数量从0到9不等，平均为3.6条。在使用11种任意选择的引物进行的家系研究中，11种引物中有5种产生了多态性条带，这些条带作为显性标记表现出孟德尔遗传。当比较纯种马和阿拉伯马的混合DNA时，我们发现10种引物能够识别出一个品种的混合DNA中存在而另一个品种的混合DNA中不存在的标记。对个体马匹的检测显示，只有两个标记在一组中完全不存在，而在另一组的成员中存在。引物UBC - 85（5'-GTGCTCGTGC-3'）检测到一对在纯种马中不存在但在31匹阿拉伯马中的11匹中存在的标记。这些标记长度分别为1500和1700碱基对（bp），分别命名为UBC - 85C和UBC - 85D。引物UBC - 126（5'-CTTTCGTGCT-3'）检测到一个1000 bp的标记（命名为UBC - 126C），在20匹纯种马中均不存在，但在31匹阿拉伯马中均存在。（摘要截短至250字）
