Bukhtiyarova M, Yang R, Ray B
Animal Science Department, University of Wyoming, Laramie 82071.
Appl Environ Microbiol. 1994 Sep;60(9):3405-8. doi: 10.1128/aem.60.9.3405-3408.1994.
The 3,500-bp pap operon in the 8,877-bp plasmid pSMB74 contains a cluster of four genes, papABCD, of which papA encodes prepediocin (A. M. Motlagh, M. Bukhtiyarova, and B. Ray, Lett. Appl. Microbiol. 18:305-312, 1994). The cluster without the promoter was cloned in the shuttle vector pHPS9. An Escherichia coli strain and a pediocin-sensitive Pediococcus acidilactici strain transformed with the recombinant plasmid, pMBR1.0, produced pediocin AcH. Deletion analysis by introducing mutations in the four genes in pMBR1.0 revealed that only papA and papD were required for pediocin AcH production and that the gene product of papD has both translocation and processing functions. In the transformed minicells of E. coli chi 925 the proteins of the pap cluster were synthesized, indicating no polar effect due to deletion.
8877 bp的质粒pSMB74中3500 bp的pap操纵子包含四个基因簇，即papABCD，其中papA编码前片球菌素（A.M.莫特拉格、M.布赫蒂亚罗娃和B.雷，《应用微生物学快报》18:305 - 312，1994年）。不含启动子的基因簇被克隆到穿梭载体pHPS9中。用重组质粒pMBR1.0转化的大肠杆菌菌株和对片球菌素敏感的嗜酸乳杆菌菌株产生了片球菌素AcH。通过在pMBR1.0的四个基因中引入突变进行缺失分析表明，片球菌素AcH的产生仅需要papA和papD，并且papD的基因产物具有转运和加工功能。在大肠杆菌chi 925的转化小细胞中合成了pap基因簇的蛋白质，表明缺失没有产生极性效应。
