Motlagh A, Bukhtiyarova M, Ray B
Department of Animal Science, University of Wyoming, Laramie 82071.
Lett Appl Microbiol. 1994 Jun;18(6):305-12. doi: 10.1111/j.1472-765x.1994.tb00876.x.
Several Pediococcus acidilactici strains produce a plasmid-encoded bacteriocin, pediocin AcH. Previous studies have shown that this plasmid, designated as pSMB 74, encodes genes associated with the production of prepediocin, its post-translation processing to pediocin AcH, transmembrane translocation of these molecules, and immunity of producer cells against pediocin AcH. We report here the complete nucleotide sequence of pSMB 74. The plasmid has a total of 8877 bp. Four genes have been located on pSMB 74. The genes are arranged in a gene cluster of 3500 bp and share a common promoter and rho-independent stem-loop terminator. The four genes, each with independent ribosome binding sites (rbs), initiation and termination codons and spacer sequences in between, were designated as pap A, pap B, pap C and pap D and encode respectively for proteins of 62, 112, 174 and 724 amino acids. The results of this study can be useful either to introduce a suitable marker at a unique restriction site in pSMB 74 and use it as a vector or to clone the pap gene cluster in a suitable plasmid and transform desirable strains for pediocin AcH production. The gene sequence has been submitted to Gene Bank (Acc. No. U02482).
几种嗜酸乳杆菌菌株可产生一种由质粒编码的细菌素——片球菌素AcH。先前的研究表明，这种名为pSMB 74的质粒编码与前片球菌素产生、其翻译后加工成片球菌素AcH、这些分子的跨膜转运以及产生菌细胞对片球菌素AcH的免疫相关的基因。我们在此报告pSMB 74的完整核苷酸序列。该质粒共有8877 bp。在pSMB 74上定位了四个基因。这些基因排列在一个3500 bp的基因簇中，共享一个共同的启动子和不依赖rho因子的茎环终止子。这四个基因，每个都有独立的核糖体结合位点（rbs）、起始和终止密码子以及其间的间隔序列，分别被命名为pap A、pap B、pap C和pap D，分别编码62、112、174和724个氨基酸的蛋白质。本研究结果可用于在pSMB 74的独特限制性位点引入合适的标记并将其用作载体，或者将pap基因簇克隆到合适的质粒中并转化所需菌株以生产片球菌素AcH。该基因序列已提交至基因库（登录号：U02482）。
