Taylor P W
J Med Microbiol. 1976 Nov;9(4):405-21. doi: 10.1099/00222615-9-4-405.
Factors that may determine the variable resistance of urinary strains of Escherichia coli to the bactericidal activity of normal human serum have been analysed. No statistically significant difference was found in the amount of lipopolysaccharide (LPS) that could be extracted from serum-sensitive and serum-resistant strains by either the phenol-water or warm-saline techniques. The ratio of LPS O-side-chain sugars to core sugars was not found to be significantly greater in serum-resistant than in serum-sensitive strains. A sugar resembling D-glycero-D-mannoheptose was found in LPS from some of the strains; in one case the sugar was shown to be associated with the O-side chain moiety. Lipopolysaccharides from all but two of the strains contained the E. coli R1 core structure. No consistent difference was observed between serum-sensitive and serum-resistant strains in either the amount of acidic polysaccharide extracted or its red-cell agglutination-inhibiting activity; nor was a clear relationship found between sensitivity to serum and sensitivity to R-specific bacteriophages. It is concluded that no one mechanism of serum resistance explains the response to serum of the E. coli strains examined in this study.
对可能决定大肠杆菌尿液菌株对正常人血清杀菌活性的可变抗性的因素进行了分析。通过酚水或温盐水技术从血清敏感菌株和血清抗性菌株中提取的脂多糖（LPS）量未发现有统计学上的显著差异。未发现血清抗性菌株中LPS O侧链糖与核心糖的比例显著高于血清敏感菌株。在一些菌株的LPS中发现了一种类似于D-甘油-D-甘露庚糖的糖；在一个案例中，该糖被证明与O侧链部分相关。除两株菌株外，所有菌株的脂多糖都含有大肠杆菌R1核心结构。在血清敏感菌株和血清抗性菌株之间，无论是提取的酸性多糖量还是其红细胞凝集抑制活性，均未观察到一致的差异；对血清的敏感性与对R特异性噬菌体的敏感性之间也未发现明显的关系。得出的结论是，没有一种血清抗性机制能够解释本研究中所检测的大肠杆菌菌株对血清的反应。
