Burns T M, Harding R M, Dale J L
Centre for Molecular Biotechnology, School of Life Science, Queensland University of Technology, Brisbane, Australia.
Arch Virol. 1994;137(3-4):371-80. doi: 10.1007/BF01309482.
A 93 nucleotide sequence was found to be strongly conserved between two ssDNA genomic components of banana bunchy top virus (BBTV). Two outwardly extending degenerate primers were designed from this sequence and used in a polymerase chain reaction (PCR) with DNA extracted from purified BBTV virions. PCR amplified products consisting of at least seven distinct bands all approximately 1 kb and possibly representing full-length BBTV dsDNA were resolved. The PCR amplified products were cloned and the clones screened by restriction enzyme analysis. Four distinct restriction analysis groups were identified. These results confirm that the genome of BBTV contains at least five components and that it belongs to a previously undescribed group of plant viruses which may also contain subterranean clover stunt virus.
在香蕉束顶病毒（BBTV）的两个单链DNA基因组组分之间发现了一段93个核苷酸的序列具有高度保守性。根据该序列设计了两条向外延伸的简并引物，并将其用于对从纯化的BBTV病毒粒子中提取的DNA进行聚合酶链反应（PCR）。PCR扩增产物由至少七条不同的条带组成，所有条带大小均约为1 kb，可能代表全长BBTV双链DNA，这些条带得到了分离。对PCR扩增产物进行克隆，并通过限制性酶切分析对克隆进行筛选。鉴定出四个不同的限制性分析组。这些结果证实，BBTV的基因组至少包含五个组分，并且它属于一类以前未描述过的植物病毒，这类病毒可能还包括三叶草矮化病毒。
