Koike G, Horiuchi M, Yamada T, Szpirer C, Jacob H J, Dzau V J
Falk Cardiovascular Research Center, Stanford University School of Medicine, CA 94305-5246.
Biochem Biophys Res Commun. 1994 Sep 30;203(3):1842-50. doi: 10.1006/bbrc.1994.2402.
The human type 2 angiotensin II receptor (AT2R) gene was isolated from a human genomic DNA library of adult female leukocytes. The putative coding region of the human AT2R gene is intronless. The primary structure of the human AT2R deduced from the nucleotide sequence of this putative coding region contains 363 amino acid residues and is highly homologous to the sequences of the rat and mouse AT2R. The human AT2R gene was mapped to the X chromosome using chromosomal assignment analysis with a human/rodent somatic cell hybrid panel. Northern blot analysis demonstrated a 3.0 kb mRNA in the human fetal lung and kidney and the human adult lung. These data and probes should enhance research in human AT2R biology.
人2型血管紧张素II受体（AT2R）基因是从成年女性白细胞的人基因组DNA文库中分离出来的。人AT2R基因的推定编码区无内含子。从该推定编码区的核苷酸序列推导的人AT2R的一级结构包含363个氨基酸残基，并且与大鼠和小鼠AT2R的序列高度同源。使用人与啮齿动物体细胞杂交板的染色体定位分析将人AT2R基因定位于X染色体。Northern印迹分析表明在人胎儿肺和肾以及成人肺中有3.0 kb的mRNA。这些数据和探针应能加强对人AT2R生物学的研究。
