Mikecz K, Glant T T
Rush Medical College, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612.
Arthritis Rheum. 1994 Sep;37(9):1395-403. doi: 10.1002/art.1780370919.
To describe the migration and homing of labeled donor lymphocytes to the lymphoid organs and synovial tissues of host animals, during the development of cartilage proteoglycan (PG)-induced arthritis adoptively transferred to syngeneic BALB/c mice.
Lymphocytes from either nonarthritic or arthritic donor animals were labeled with either fluorescent or radioactive cell linkers (PKH-GL) and injected into syngeneic, immunosuppressed mice. The homing patterns of donor lymphocytes following the injection of labeled cells were studied by fluorescence microscopy and by measurement of radioactivity in tissue samples.
Lymphocytes from arthritic donors retained their ability to transfer the disease after labeling. In the lymphoid organs, arthritic and nonarthritic donor lymphocytes exhibited similar homing patterns, although remarkable differences were found in the number of homing cells derived from nonarthritic and arthritic donors. However, labeled cells only from arthritic animals migrated to the synovial tissue of the recipient mice, and their appearance was associated with the onset of arthritis.
Lymphocytes from mice with PG-induced arthritis, in contrast to lymphocytes from non-arthritic donors, exhibit preferential homing to the synovial tissue of the host. Adoptive transfer of arthritis is linked to the appearance of these cells in the synovium.
描述在将软骨蛋白聚糖（PG）诱导的关节炎过继转移至同基因BALB/c小鼠的过程中，标记的供体淋巴细胞向宿主动物的淋巴器官和滑膜组织的迁移与归巢情况。
来自非关节炎或关节炎供体动物的淋巴细胞用荧光或放射性细胞连接剂（PKH-GL）标记，然后注射到同基因、免疫抑制的小鼠体内。通过荧光显微镜和测量组织样本中的放射性来研究注射标记细胞后供体淋巴细胞的归巢模式。
来自关节炎供体的淋巴细胞在标记后仍保留其转移疾病的能力。在淋巴器官中，关节炎和非关节炎供体淋巴细胞表现出相似的归巢模式，尽管在源自非关节炎和关节炎供体的归巢细胞数量上发现了显著差异。然而，只有来自关节炎动物的标记细胞迁移到受体小鼠的滑膜组织，并且它们的出现与关节炎的发作相关。
与来自非关节炎供体的淋巴细胞相比，PG诱导的关节炎小鼠的淋巴细胞表现出优先归巢至宿主的滑膜组织。关节炎的过继转移与这些细胞在滑膜中的出现有关。
