Duan H J, Gao F, Oguchi K, Nagata T
Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, Japan.
Arzneimittelforschung. 1994 Jul;44(7):880-3.
The aim of this study was to investigate whether the water particles produced by a nebulizer can reach the alveoli of the lung when they are inhaled with the air. For the purpose of demonstrating water particle incorporation into the lung, light and electron microscopic radioautography with tritiated thymidine was employed. Tritiated thymidine was dissolved in distilled water and nebulized to fine water particles with a new type nebulizer named Shinki, then inhaled by mice. The lungs were taken out and processed by either rapid-freezing and freeze-substitution for dry-mounting radioautography or conventional chemical fixation for wet-mounting radioautography. By wet-mounting radioautography, silver grains were observed in the nuclei of a few alveolar type 2 cells and interstitial cells, demonstrating DNA synthesis. By dry-mounting radioautography numerous silver grains were located diffusely in all the epithelial and interstitial cells, demonstrating diffuse localization of soluble radioisotope-labeled compounds. These results show that water particles produced by the Shinki nebulizer can directly reach the alveoli and thus, better therapeutic effect can be acquired with the Shinki nebulizer than with the usual nebulizer. Further, the nebulizer can be used for the radioautographic studies of the lung in demonstrating incorporation of some drugs into alveolar tissues.
本研究的目的是调查雾化器产生的水颗粒与空气一同被吸入时是否能抵达肺的肺泡。为了证明水颗粒进入肺部，采用了用氚化胸腺嘧啶进行光镜和电镜放射自显影术。将氚化胸腺嘧啶溶解于蒸馏水中，并用一种名为“新基”的新型雾化器将其雾化成细小的水颗粒，然后让小鼠吸入。取出肺脏，通过快速冷冻和冷冻置换进行干装放射自显影处理，或通过传统化学固定进行湿装放射自显影处理。通过湿装放射自显影，在少数Ⅱ型肺泡细胞和间质细胞的细胞核中观察到银颗粒，表明有DNA合成。通过干装放射自显影，大量银颗粒弥散分布于所有上皮细胞和间质细胞中，表明可溶性放射性同位素标记化合物的弥散定位。这些结果表明，“新基”雾化器产生的水颗粒可直接抵达肺泡，因此，与普通雾化器相比，“新基”雾化器可获得更好的治疗效果。此外，该雾化器可用于肺部的放射自显影研究，以证明某些药物进入肺泡组织的情况。
